Neurochemical Research

, Volume 43, Issue 3, pp 650–658 | Cite as

Ischemia–Reperfusion Injury of Sciatic Nerve in Rats: Protective Role of Combination of Vitamin C with E and Tissue Plasminogen Activator

  • Katerina Apostolopoulou
  • Dimitris Konstantinou
  • Rodoula Alataki
  • Ioannis Papapostolou
  • Dimitrios Zisimopoulos
  • Electra Kalaitzopoulou
  • Vasiliki Bravou
  • Ioannis Lilis
  • Fevronia Angelatou
  • Helen Papadaki
  • Christos D. Georgiou
  • Elisabeth Chroni
Original Paper


An ischemia/reperfusion injury of rat’s sciatic nerve was experimentally developed. In this model, we measured the in vivo production of superoxide radical, as a marker of oxidative stress and the occludin expression as an indicator of blood-nerve barrier function and we examined potential protective innervations against these abnormalities. Right sciatic nerves of the animals underwent 3 h of ischemia followed by 7 days of reperfusion and were divided into three groups: ischemic, pretreated with vitamin C in conjunction with vitamin E and treated with tissue plasminogen activator. Compared to measurements from left sciatic nerves used as sham, the ischemic group showed significantly increased superoxide radical and reduced expression of occludin in western blot and immunohistochemistry. No such differences were detected between sham and nerves in the vitamin or tissue plasminogen activator groups. It is suggested that the experimental ischemia/reperfusion model was suitable for studying the relationship between oxidative state and blood-nerve barrier. The reversion of abnormalities by the applied neuroprotective agents might prove to be a clinically important finding in view of the implication of vascular supply derangement in various neuropathies in humans.


Peripheral nerve injury Oxidative stress Occludin Ascorbic acid A-tocopherol Tissue plasminogen activator 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Katerina Apostolopoulou
    • 1
  • Dimitris Konstantinou
    • 1
  • Rodoula Alataki
    • 2
  • Ioannis Papapostolou
    • 3
  • Dimitrios Zisimopoulos
    • 3
  • Electra Kalaitzopoulou
    • 3
  • Vasiliki Bravou
    • 4
  • Ioannis Lilis
    • 4
  • Fevronia Angelatou
    • 2
  • Helen Papadaki
    • 4
  • Christos D. Georgiou
    • 3
  • Elisabeth Chroni
    • 5
  1. 1.Department of Neurosurgery, School of MedicineUniversity of PatrasPatrasGreece
  2. 2.Department of Physiology, School of MedicineUniversity of PatrasPatrasGreece
  3. 3.Department of BiologyUniversity of PatrasPatrasGreece
  4. 4.Department of Anatomy, School of MedicineUniversity of PatrasPatrasGreece
  5. 5.Department of Neurology, School of MedicineUniversity of PatrasRio-PatrasGreece

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