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Soy Isoflavone Antagonizes the Oxidative Cerebrovascular Injury Induced by β-Amyloid Peptides 1–42 in Rats

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Abstract

Numerous evidences have shown that the antioxidative properties of soy isoflavone (SIF) have beneficial effects on prophylaxis of neurodegeneration, however, the mechanism is still not fully illustrated. As cerebrovascular dysfunction could initiate a cascade of events leading to pathogenesis of Alzheimer’s disease, we tried to investigate whether SIF could protect the cerebrovascular system due to antagonizing oxidative damage induced by Aβ1–42 in present study. In addition, NF-E2-related factor 2 (Nrf2) signaling pathways in the cerebrovascular tissue of Wistar rats were investigated to identify the potential cerebrovascular protective targets of SIF. Research results showed that SIF reduced the excessive production of nitrotyrosine in cerebrovascular tissue induced by Aβ1–42, and maintained redox homeostasis by increasing the level of GSH and GSH/GSSG. Moreover, SIF could alleviate the down-regulation of Nrf2, γ-glutamylcysteine synthetase, Heme oxygenase-1 expressions in cerebrovascular tissue induced by Aβ1–42 and suppress the increase of Kelch like ECH protein-1 (Keap1). These data suggested that SIF might reduce the cerebrovascular oxidative damage induced by Aβ1–42 through regulating the Nrf2 signaling pathway. The mechanisms of SIF modulating the potential target Nrf2 might be associated with Keap1 expression.

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Abbreviations

Aβ:

β-Amyloid peptides

AD:

Alzheimer’s disease

Nrf2:

Transcription factor NF-E2-related factor2

Keap1:

Kelch like ECH protein-1

γ-GCS:

γ-Glutamylcysteine synthetase

HO-1:

Heme oxygenase-1

GSH/GSSG:

Reduced glutathione/oxidized glutathione

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Acknowledgments

The authors appreciate the financial support provided by National Natural Science Foundation of China (No. 81172661 and 81302427), the National High Technology Research and Development Program (863 Program) of China (No. 2010AA023003).

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. School of Public Health, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing, People’s Republic of China

    Yuan-Di Xi, Huan-Ling Yu, Han Jing, Wei-Wei Ma, Lin-Hong Yuan, Dan-Di Zhang & Rong Xiao

  2. Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Beijing, People’s Republic of China

    Xiao-Ying Li & Jian Wu

  3. Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People’s Republic of China

    Yuan-Di Xi, Huan-Ling Yu, Han Jing, Wei-Wei Ma, Lin-Hong Yuan, Dan-Di Zhang & Rong Xiao

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  1. Yuan-Di Xi
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  2. Xiao-Ying Li
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  3. Huan-Ling Yu
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  4. Han Jing
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  7. Dan-Di Zhang
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  8. Jian Wu
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Corresponding author

Correspondence to Rong Xiao.

Additional information

Yuan-Di Xi and Xiao-Ying Li have contributed equally to this work.

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Xi, YD., Li, XY., Yu, HL. et al. Soy Isoflavone Antagonizes the Oxidative Cerebrovascular Injury Induced by β-Amyloid Peptides 1–42 in Rats. Neurochem Res 39, 1374–1381 (2014). https://doi.org/10.1007/s11064-014-1319-x

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  • DOI: https://doi.org/10.1007/s11064-014-1319-x

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