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Anti-inflammatory Effect of Tanshinone I in Neuroprotection Against Cerebral Ischemia–Reperfusion Injury in the Gerbil Hippocampus

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Abstract

Tanshinone I (TsI) is an important lipophilic diterpene extracted from Danshen (Radix Salvia miltiorrhizae) and has been used in Asia for the treatment of cerebrovascular diseases such as ischemic stroke. In this study, we examined the neuroprotective effect of TsI against ischemic damage and its neuroprotective mechanism in the gerbil hippocampal CA1 region (CA1) induced by 5 min of transient global cerebral ischemia. Pre-treatment with TsI protected pyramidal neurons from ischemic damage in the stratum pyramidale (SP) of the CA1 after ischemia–reperfusion. The pre-treatment with TsI increased the immunoreactivities and protein levels of anti-inflammatory cytokines [interleukin (IL)-4 and IL-13] in the TsI-treated-sham-operated-groups compared with those in the vehicle-treated-sham-operated-groups; however, the treatment did not increase the immunoreactivities and protein levels of pro-inflammatory cytokines (IL-2 and tumor necrosis factor-α). On the other hand, in the TsI-treated-ischemia-operated-groups, the immunoreactivities and protein levels of all the cytokines were maintained in the SP of the CA1 after transient cerebral ischemia. In addition, we examined that IL-4 injection into the lateral ventricle did not protect pyramidal neurons from ischemic damage. In conclusion, these findings indicate that the pre-treatment with TsI can protect against ischemia-induced neuronal death in the CA1 via the increase or maintenance of endogenous inflammatory cytokines, and exogenous IL-4 does not protect against ischemic damage.

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Acknowledgments

The authors would like to thank Mr. Seung Uk Lee for his technical help in this study. This research was supported by 2013 Research Grant from Kangwon National University (12013-1867) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2011-0013933).

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Authors and Affiliations

  1. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Joon Ha Park, Jeong-Hwi Cho, In Hye Kim, Ji Hyeon Ahn, Jae-Chul Lee & Moo-Ho Won

  2. Division of Analytical Bio-imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon, 200-701, South Korea

    Ok kyu Park & Seung-Hae Kwon

  3. Department of Physiology, Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea

    Bai Hui Chen & Yun Lyul Lee

  4. Institute of Integrative Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou, 225001, China

    Bing Chun Yan

  5. Department of Oral Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung, 210-702, South Korea

    Ki-Yeon Yoo

  6. Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, 330-714, South Korea

    Choong Hyun Lee

  7. Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea

    In Koo Hwang

  8. Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Jung Hoon Choi

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  1. Joon Ha Park
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Correspondence to Moo-Ho Won or Jung Hoon Choi.

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Joon Ha Park and Ok kyu Park have contributed equally to this article.

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Park, J.H., Park, O.k., Cho, JH. et al. Anti-inflammatory Effect of Tanshinone I in Neuroprotection Against Cerebral Ischemia–Reperfusion Injury in the Gerbil Hippocampus. Neurochem Res 39, 1300–1312 (2014). https://doi.org/10.1007/s11064-014-1312-4

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  • DOI: https://doi.org/10.1007/s11064-014-1312-4

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