Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium
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Umbilical cord blood-derived marrow stromal cells (UCB-MSCs) with high proliferation capacity and immunomodulatory properties are considered to be a good candidate for cell-based therapies. But until now, little work has been focused on the differentiation of UCB-MSCs. In this work, UCB-MSCs were demonstrated to be negative for CD34 and CD45 expression but positive for CD90 and CD105 expression. The gate values of UCB-MSCs for CD90 and CD105 were 99.3 and 98.6 %, respectively. Two weeks after treatment, the percentage of neuron-like cells differentiated from UCB-MSCs was increased to 84 ± 12 % in the experimental group [treated with olfactory ensheathing cells (OECs)-conditioned medium] and they were neuron-specific enolase positive; few neuron-like cells were found in the control group (without OECs-conditioned medium). Using whole-cell recording, sodium and potassium currents were recorded in UCB-MSCs after differentiation by OECs. Thus, human UCB-MSCs could be differentiated to neural cells by secreted secretion from OECs and exhibited electrophysiological properties similar to mature neurons after 2 weeks post-induction. These results imply that OECs can be used as a new strategy for stem cell differentiation and provide an alternative neurogenesis pathway for generating sufficient numbers of neural cells for cell therapy.
KeywordsStem cell Differentiation Olfactory ensheathing cells Ion channel
Conflict of interest
The authors declare no conflict of interest.
- 3.Capelli C, Gotti E, Morigi M, Rota C, Weng L, Dazzi F, Spinelli O, Cazzaniga G, Trezzi R, Gianatti A, Rambaldi A, Golay J, Introna M (2011) Minimally manipulated whole human umbilical cord is a rich source of clinical-grade human mesenchymal stromal cells expanded in human platelet lysate. Cytotherapy 13:786–801PubMedCrossRefGoogle Scholar
- 12.Koch TG, Heerkens T, Thomsen PD, Betts DH (2007) Isolation of mesenchymal stem cells from equine umbilical cord blood, BMC. Biotechnology 7:1–9Google Scholar
- 15.Lee HJ, Lee JK, Lee H, Carter JE, Chang WJ, Oh W, Yang YS, Suh JG, Lee BH, Jin HK, Bae JS (2012) Human umbilical cord blood-derived mesenchymal stem cells improve neuropathology and cognitive impairment in an Alzheimer’s disease mouse model through modulation of neuroinflammation. Neurobiol Aging 33:588–602PubMedCrossRefGoogle Scholar
- 16.Liu Y, Teng X, Yang X, Song Q, Lu R, Xiong J, Liu B, Zeng N, Zeng Y, Long J, Cao R, Lin Y, He Q, Chen P, Lu M, Liang S (2010) Shotgun proteomics and network analysis between plasma membrane and extracellular matrix proteins from rat olfactory ensheathing cells. Cell Transpl 19:133–146CrossRefGoogle Scholar
- 19.Park SI, Lim JY, Jeong CH, Kim SM, Jun JA, Jeun SS, Oh WI (2012) Human umbilical cord blood-derived mesenchymal stem cell therapy promotes functional recovery of contused rat spinal cord through enhancement of endogenous cell proliferation and oligogenesis. J Biomed Biotechnol 2012:362473PubMedGoogle Scholar
- 20.Peters R, Wolf MJ, van den Broek M, Nuvolone M, Dannenmann S, Stieger B, Rapold R, Konrad D, Rubin A, Bertino JR, Aguzzi A, Heikenwalder M, Knuth AK (2010) Efficient generation of multipotentmesenchymal stem cells from umbilical cord blood in stroma-free liquid culture. PLoS One 5:e15689PubMedCrossRefGoogle Scholar
- 23.Rao MS, Mattson MP (2001) Stem cells and aging: expanding the possibilities. Mech Ageing Dev 1227:13–734Google Scholar
- 27.Srivastava N, Seth K, Khanna VK, Ansari RW, Agrawal AK (2009) Long-term functional restoration by neural progenitor cell transplantation in rat model of cognitive dysfunction: co-transplantation with olfactory ensheathing cells for neurotrophic factor support. Int J Dev Neurosci 27:103–110PubMedCrossRefGoogle Scholar