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Melatonin Protects Against Diazinon-Induced Neurobehavioral Changes in Rats

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Abstract

Diazinon is an organophosphorous pesticide with a prominent toxicity on many body organs. Multiple mechanisms contribute to diazinon-induced deleterious effects. Inhibition of acetyl-cholinesterase, cholinergic hyperstimulation, and formation of reactive oxygen species may play a role. On the other hand, melatonin is a pineal hormone with a well-known potent antioxidant activity and a remarkable modulatory effect on many behavioral processes. The present study revealed that oral diazinon administration (25 mg/kg) increased anxiety behavior in rats subjected to elevated plus maze and open-field tests possibly via the induction of changes in brain monoamines levels (dopamine, norepinephrine, and serotonin). Additionally, brain lipid peroxides measured as malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) levels were elevated, while the activity of brain glutathione peroxidase enzyme was reduced by diazinon. Co-administration of oral melatonin (10 mg/kg) significantly attenuated the anxiogenic activity of diazinon, rebalanced brain monoamines levels, decreased brain MDA and TNF-α levels, and increased the activity of brain glutathione peroxidase enzyme.

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The authors declare that they have no conflict of interest and that they have contributed equally in every part of this work.

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  1. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Misr University for Science and Technology (MUST), 6th of October City, Egypt

    Maha A. E. Ahmed

  2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University (Girls), Nasr City, Egypt

    Hebatalla I. Ahmed

  3. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Helwan University, Ein Helwan, Helwan, Egypt

    Engy M. El-Morsy

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  1. Maha A. E. Ahmed
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Ahmed, M.A.E., Ahmed, H.I. & El-Morsy, E.M. Melatonin Protects Against Diazinon-Induced Neurobehavioral Changes in Rats. Neurochem Res 38, 2227–2236 (2013). https://doi.org/10.1007/s11064-013-1134-9

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