Neurochemical Research

, Volume 38, Issue 3, pp 601–609 | Cite as

The Molecular Events Involved in Oligodendrocyte Precursor Cell Proliferation Induced by the Conditioned Medium from B104 Neuroblastoma Cells

  • Jian-Guo Hu
  • Xing-Jun Wu
  • Yi-Fan Feng
  • Gang-ming Xi
  • Ling-Xiao Deng
  • Zhen-Huan Wang
  • Rui Wang
  • Lin Shen
  • Jian-Sheng Zhou
  • He-Zuo Lü
Original Paper


The conditioned medium from B104 neuroblastoma cells (B104CM) induces proliferation of oligodendrocyte progenitor cells (OPCs) in vitro. However, the molecular events that occur during B104CM-induced proliferation of OPCs has not been well clarified. In the present study, using OPCs immunopanned from embryonic day 14 Sprague–Dawley rat spinal cords, we explored the activation of several signaling pathways and the expression of several important immediate early genes (IEGs) and cyclins in OPCs in response to B104CM. We found that B104CM can induce OPC proliferation through the activation of the extracellular signal-regulated kinases 1 and 2 (Erk1/2), but not PI3K or p38 MAPK signaling pathways in vitro. The IEGs involved in B104CM-induced OPC proliferation include c-fos, c-jun and Id2, but not c-myc, fyn, or p21. The cyclins D1, D2 and E are also involved in B104CM-stimulated proliferation of OPCs. The activation of Erk results in subsequent expression of IEGs (such as c-fos, c-jun and Id-2) and cyclins (including cyclin D1, D2 and E), which play key roles in cell cycle initiation and OPC proliferation. Collectively, these results suggest that the phosphorylation of Erk1/2 is an important molecular event during OPC proliferation induced by B104CM.


B104CM OPCs Proliferation Erk Immediate early genes Cyclins 



This work was supported by the National Natural Science Foundation of China (NO. 81071268; 81171465), the Key Project of Chinese Ministry of Education (NO. 210103).

Conflict of interest

The authors have declared no conflict of interest statement.


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Jian-Guo Hu
    • 1
    • 2
  • Xing-Jun Wu
    • 3
  • Yi-Fan Feng
    • 1
    • 2
  • Gang-ming Xi
    • 3
  • Ling-Xiao Deng
    • 4
  • Zhen-Huan Wang
    • 2
  • Rui Wang
    • 2
  • Lin Shen
    • 2
  • Jian-Sheng Zhou
    • 2
  • He-Zuo Lü
    • 1
    • 2
  1. 1.Department of Clinical Laboratory ScienceThe First Affiliated Hospital of Bengbu Medical CollegeBengbuPeople’s Republic of China
  2. 2.Anhui Key Laboratory of Tissue TransplantationBengbu Medical CollegeBengbuPeople’s Republic of China
  3. 3.Department of NeurologyXuhui Central HospitalShanghaiPeople’s Republic of China
  4. 4.Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute and Departments of Neurological Surgery and Anatomy and Cell BiologyIndiana University School of MedicineIndianapolisUSA

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