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Neurochemical Research

, Volume 38, Issue 3, pp 530–537 | Cite as

Propofol Increases Expression of Basic Fibroblast Growth Factor After Transient Cerebral Ischemia in Rats

  • Xiao-Chun Zhao
  • Li-Min Zhang
  • Dong-Yi Tong
  • Ping An
  • Chao Jiang
  • Ping Zhao
  • Wei-Min Chen
  • Jian Wang
Original Paper

Abstract

Anesthetics such as propofol can provide neuroprotective effects against cerebral ischemia. However, the underlying mechanism of this beneficial effect is not clear. Therefore, we subjected male Sprague–Dawley rats to 2 h of middle cerebral artery occlusion and investigated how post-ischemic administration of propofol affected neurologic outcome and the expression of basic fibroblast growth factor (bFGF). After 2 h of ischemia, just before reperfusion, the animals were randomly assigned to receive either propofol (20 mg kg−1 h−1) or vehicle (10 % intralipid, 2 ml kg−1 h−1) intravenously for 4 h. Neurologic scores, infarct volume, and brain water content were measured at different time points after reperfusion. mRNA level of bFGF was measured by real-time PCR, and the protein expression level of bFGF was analyzed by immunohistochemistry and Western blot. At 6, 24, 72 h, and 7 days of reperfusion, infarct volume was significantly reduced in the propofol-treated group compared to that in the vehicle-treated group (all P < 0.05). Propofol post-treatment also attenuated brain water content at 24 and 72 h and reduced neurologic deficit score at 72 h and 7 days of reperfusion (all P < 0.05). Additionally, in the peri-infarct area, bFGF mRNA and protein expression were elevated at 6, 24, and 72 h of reperfusion compared to that in the vehicle-treated group (all P < 0.05). These results show that post-ischemic administration of propofol provides neural protection from cerebral ischemia–reperfusion injury. This protection may be related to an early increase in the expression of bFGF.

Keywords

Brain Basic fibroblast growth factor Ischemia–reperfusion Propofol 

Notes

Acknowledgments

This work was supported by NSFC (81000824, 81101402, 81171782), and NIH (K01AG031926, R01AT007317, R01NS078026). We thank Claire Levine for assistance with this manuscript.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Xiao-Chun Zhao
    • 1
  • Li-Min Zhang
    • 1
  • Dong-Yi Tong
    • 1
  • Ping An
    • 2
  • Chao Jiang
    • 3
  • Ping Zhao
    • 1
  • Wei-Min Chen
    • 1
  • Jian Wang
    • 4
  1. 1.Department of AnesthesiologyShengJing Hospital of China Medical UniversityShenyangChina
  2. 2.Department of Neurobiology, College of Basic MedicineChina Medical UniversityShenyangChina
  3. 3.Department of NeurologyFifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina
  4. 4.Department of Anesthesiology and Critical Care Medicine, School of MedicineJohns Hopkins UniversityBaltimoreUSA

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