Arsenosugar Induced Blood and Brain Oxidative Stress, DNA Damage and Neurobehavioral Impairments
- 294 Downloads
The effect of Arsenosugar on motor function and contextual memory-related to place and event; the extent of DNA damage and oxidative stress in male swiss albino mice was investigated. Passive avoidance test was used for memory test; rota motor test was used for motor function. Several biochemical parameters were used for assessing oxidative stress due to arsenosugar ingestion. Decreased passive avoidance time and decreased retention time in rotating rod indicated disruption of normal neurobehavior. Significant dose-dependent DNA damage was found in mice blood and brain. Decreased super oxide dismutase, increased lipid peroxidation, decreased protein sulfohydryl content, increased protein carbonyl content in blood and hippocampal tissue; glutathione in blood and glutathione peroxidase in hippocampal tissue indicated the ability of arsenosugar to cause oxidative stress. This study concludes with evidence that arsenosugar ingestion causes higher oxidative stress, increases DNA damage in the blood and hippocampus in vivo. This might be responsible for the dysfunction of cognitive and motor functions. However, further investigation is suggested for deciphering the biomolecular mechanism.
KeywordsArsenosugar Passive avoidance test Rota motor test Oxidative stress Comet assay
The first and corresponding author (Muhammad Shahdaat Bin Sayeed) was awarded travel grant for presenting partial result of the study in the 35th Annual Meeting of Japan Neuroscience Society, 18-21 September, 2012 in Nagoya, Japan.
Conflict of interest
- 1.Shimbo S, Hayase A, Murakami M, Hatai I, Higashikawa K, Moon CS, Zhang ZW, Watanabe T, Iguchi H, Ikeda M (1996) Use of a food composition database to estimate daily dietary intake of nutrient or trace elements in Japan, with reference to its limitation. Food Addit Contam 13:775–786PubMedCrossRefGoogle Scholar
- 4.WHO (1989) Evaluation of certain food additives and contaminants; 33rd Report of the Joint FAO/WHO Expert Committee on Food Additives; WHO: GenevaGoogle Scholar
- 12.World Medical Association Declaration of Helsinki. Ethical Principles for Medical Research Involving Human Subjects. Adopted by the 18th WMA General Assembly, Helsinki, Finland, June 1964, and amended by the 59th WMA General Assembly Seoul, South Korea, October, 2008. http://www.wma.net/en/30publications/10policies/b3/index.html. Accessed 1 August, 2012
- 15.Dunham NW, Meya TS (1957) A note on simple apparatus for detecting neurological defects in rats and mice. J Am Pharm Assoc 46:208–209Google Scholar
- 25.Klaassen CD, Watkins JB (2003) Casarett and Doull’s essentials of toxicology. McGraw-Hill: New York. p 512. ISBN. 978-0-07-138914–3Google Scholar
- 30.Warburton DM (1975) Brain, behaviour and drugs. Wiley, LondonGoogle Scholar
- 33.Münch G, Deuther-Conrad W, Gasic-Milenkovic J (2002) Glycoxidative stress creates a vicious cycle of neurodegeneration in alzheimer’s disease–a target for neuroprotective treatment strategies? J Neural Transm Suppl 620:303–307Google Scholar