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Neurochemical Research

, Volume 38, Issue 2, pp 405–412 | Cite as

Arsenosugar Induced Blood and Brain Oxidative Stress, DNA Damage and Neurobehavioral Impairments

  • Muhammad Shahdaat Bin Sayeed
  • Md. Ratan
  • Farhad Hossen
  • Faizule Hassan
  • Mohammad Faisal
  • Mohammad Fahim Kadir
Original Paper

Abstract

The effect of Arsenosugar on motor function and contextual memory-related to place and event; the extent of DNA damage and oxidative stress in male swiss albino mice was investigated. Passive avoidance test was used for memory test; rota motor test was used for motor function. Several biochemical parameters were used for assessing oxidative stress due to arsenosugar ingestion. Decreased passive avoidance time and decreased retention time in rotating rod indicated disruption of normal neurobehavior. Significant dose-dependent DNA damage was found in mice blood and brain. Decreased super oxide dismutase, increased lipid peroxidation, decreased protein sulfohydryl content, increased protein carbonyl content in blood and hippocampal tissue; glutathione in blood and glutathione peroxidase in hippocampal tissue indicated the ability of arsenosugar to cause oxidative stress. This study concludes with evidence that arsenosugar ingestion causes higher oxidative stress, increases DNA damage in the blood and hippocampus in vivo. This might be responsible for the dysfunction of cognitive and motor functions. However, further investigation is suggested for deciphering the biomolecular mechanism.

Keywords

Arsenosugar Passive avoidance test Rota motor test Oxidative stress Comet assay 

Notes

Acknowledgments

The first and corresponding author (Muhammad Shahdaat Bin Sayeed) was awarded travel grant for presenting partial result of the study in the 35th Annual Meeting of Japan Neuroscience Society, 18-21 September, 2012 in Nagoya, Japan.

Conflict of interest

None.

References

  1. 1.
    Shimbo S, Hayase A, Murakami M, Hatai I, Higashikawa K, Moon CS, Zhang ZW, Watanabe T, Iguchi H, Ikeda M (1996) Use of a food composition database to estimate daily dietary intake of nutrient or trace elements in Japan, with reference to its limitation. Food Addit Contam 13:775–786PubMedCrossRefGoogle Scholar
  2. 2.
    Hansen HR, Raab A, Francesconi KA, Feldmann J (2003) Metabolism of arsenic by sheep chronically exposed to arseno-sugars as a normal part of their diet: quantitative intake, uptake and excretion. Environ Sci Technol 37:845–851PubMedCrossRefGoogle Scholar
  3. 3.
    Andrewes P, Demarini DM, Funasaka K, Wallace K, Lai VW, Sun H, Cullen WR, Kitchin KT (2004) Do arsenosugars pose a risk to human health? the comparative toxicities of a trivalent and pentavalent arsenosugar. Environ Sci Technol 38:4140–4148PubMedCrossRefGoogle Scholar
  4. 4.
    WHO (1989) Evaluation of certain food additives and contaminants; 33rd Report of the Joint FAO/WHO Expert Committee on Food Additives; WHO: GenevaGoogle Scholar
  5. 5.
    Francesconi KA, Tanggaar R, McKenzie CJ, Goessler W (2002) Arsenic metabolites in human urine after ingestion of an arsenosugar. Clin Chem 48:92–101PubMedGoogle Scholar
  6. 6.
    Ma M, Le XC (1998) Effect of arsenosugar ingestion on urinary arsenic speciation. Clin Chem 44:539–550PubMedGoogle Scholar
  7. 7.
    Le XC, Cullen WR, Reimer KJ (1994) Human urinary arsenic excretion after one-time ingestion of seaweed, crab, and shrimp. Clin Chem 40:617–624PubMedGoogle Scholar
  8. 8.
    Wei C, Li W, Zhang C, Van Hulle M, Cornelis R, Zhang X (2003) Safety evaluation of organoarsenical species in edible porphyra from the China Sea. J Agric Food Chem 51:5176–5182PubMedCrossRefGoogle Scholar
  9. 9.
    Erickson BE (2003) New concerns about arsenosugars in seaweed and shellfish. Environ Sci Technol 37:84ACrossRefGoogle Scholar
  10. 10.
    Koch I, McPherson K, Smith P, Easton L, Doe KG, Reimer KJ (2007) Arsenic bioaccessibility and speciation in clams and seaweed from a contaminated marine environment. Mar Pollut Bull 54:586–594PubMedCrossRefGoogle Scholar
  11. 11.
    Ha SH, Yeun JH, Kim J, Joo JD, Lee LY (2009) New synthetic method of natural arsenosugar. Bull Korean Chem Soc 30:997–998CrossRefGoogle Scholar
  12. 12.
    World Medical Association Declaration of Helsinki. Ethical Principles for Medical Research Involving Human Subjects. Adopted by the 18th WMA General Assembly, Helsinki, Finland, June 1964, and amended by the 59th WMA General Assembly Seoul, South Korea, October, 2008. http://www.wma.net/en/30publications/10policies/b3/index.html. Accessed 1 August, 2012
  13. 13.
    Piala JJ, High JP, Hessert JLJ, Burke JC, Crower BN (1959) Pharmacological and acute toxicological comparisons of trifluoropromazine and chlorpromazine. J Pharmacol Exp Ther 127:55–65PubMedGoogle Scholar
  14. 14.
    Kuribara H, Higuchi Y, Tadokoro S (1977) Effects of central depressants on rotarod and tractionperformances in mice. Jpn J Pharmacol 27:117–126PubMedCrossRefGoogle Scholar
  15. 15.
    Dunham NW, Meya TS (1957) A note on simple apparatus for detecting neurological defects in rats and mice. J Am Pharm Assoc 46:208–209Google Scholar
  16. 16.
    Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191PubMedCrossRefGoogle Scholar
  17. 17.
    Nadin SB, Vargas-Roig LM, Ciocca DR (2001) A silver staining method for single cell gel assay. J Histochem Cytochem 49:1183–1186PubMedCrossRefGoogle Scholar
  18. 18.
    Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287PubMedCrossRefGoogle Scholar
  19. 19.
    Samir M, el-Kholy NM (1999) Thiobarbituric acid reactive substances in patients with laryngeal cancer. Clin Otolaryngol Allied Sci 24:232–234PubMedCrossRefGoogle Scholar
  20. 20.
    Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358PubMedCrossRefGoogle Scholar
  21. 21.
    Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77PubMedCrossRefGoogle Scholar
  22. 22.
    Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG et al (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464–478PubMedCrossRefGoogle Scholar
  23. 23.
    Flora SJS, Bhadauria S, Panta SC, Dhaked RK (2005) Arsenic induced blood and brain oxidative stress and its response to some thiol chelators in rats. Life Sci 77:2324–2337PubMedCrossRefGoogle Scholar
  24. 24.
    Jollow DJ, Mitchell JR, Zamppaglione Z, Gillette JR (1974) Bromobenzene induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolites. Pharmacology 11:151–157PubMedCrossRefGoogle Scholar
  25. 25.
    Klaassen CD, Watkins JB (2003) Casarett and Doull’s essentials of toxicology. McGraw-Hill: New York. p 512. ISBN. 978-0-07-138914–3Google Scholar
  26. 26.
    McAdam DP, Perera AMA, Stick RV (1987) The synthesis of (R)-2′,3′-dihydroxypropyl 5-deoxy-5-dimethylarsinyl-β-D-riboside, a naturally occurring arsenic-containing carbohydrate. Aust J Chem 40:1901–1908CrossRefGoogle Scholar
  27. 27.
    Stick RV, Stubbs KA, Tilbrook DMG (2001) An improved synthesis of (R)-2,3-dihydroxypropyl 5-deoxy-5-dimethylarsinyl-β-D-riboside, a common marine arsenical. Aust J Chem 54:181–183CrossRefGoogle Scholar
  28. 28.
    Cullen WR, McBride BC, Reglinski J (1984) The reduction of trimethylarsine oxide to trimethylarsine by thiols: a mechanistic model for the biological reduction of arsenical. J Inorg Biochem 21:45–60CrossRefGoogle Scholar
  29. 29.
    Kannan GM, Tripathi N, Dube SN, Gupta M, Flora SJ (2001) Toxic effects of arsenic (III) on some hematopoietic and central nervous system variables in rats and guinea pigs. J Toxicol Clin Toxicol 39:675–682PubMedCrossRefGoogle Scholar
  30. 30.
    Warburton DM (1975) Brain, behaviour and drugs. Wiley, LondonGoogle Scholar
  31. 31.
    Jomova K, Jenisova Z, Feszterova M, Baros S, Liska J, Hudecova D, Rhodesd CJ, Valko M (2011) Arsenic: toxicity, oxidative stress and human disease. J Appl Toxicol 31:95–107PubMedGoogle Scholar
  32. 32.
    Münch G, Mayer S, Michaelis J, Hipkiss AR, Riederer P, Müller R, Neumann A, Schinzel R, Cunningham AM (1997) Influence of advanced glycation end-products and AGE-inhibitors on nucleation-dependent polymerization of beta-amyloid peptide. Biochim Biophys Acta 1360:17–29PubMedCrossRefGoogle Scholar
  33. 33.
    Münch G, Deuther-Conrad W, Gasic-Milenkovic J (2002) Glycoxidative stress creates a vicious cycle of neurodegeneration in alzheimer’s disease–a target for neuroprotective treatment strategies? J Neural Transm Suppl 620:303–307Google Scholar
  34. 34.
    Gong G, O’Bryant SE (2010) The arsenic exposure hypothesis for alzheimer disease. Alzheimer Dis Assoc Disord 24:311–316CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Muhammad Shahdaat Bin Sayeed
    • 1
  • Md. Ratan
    • 2
  • Farhad Hossen
    • 1
  • Faizule Hassan
    • 3
    • 4
  • Mohammad Faisal
    • 3
    • 6
  • Mohammad Fahim Kadir
    • 5
  1. 1.Department of Clinical Pharmacy and PharmacologyUniversity of DhakaDhakaBangladesh
  2. 2.Department of Pharmaceutical ChemistryUniversity of DhakaDhakaBangladesh
  3. 3.Department of Biochemistry and Molecular BiologyUniversity of DhakaDhakaBangladesh
  4. 4.Department of Chemistry and BiochemistryMiami UniversityOxfordUSA
  5. 5.Department of Pharmaceutical TechnologyUniversity of DhakaDhakaBangladesh
  6. 6.Department of Biological SciencesLouisiana State UniversityBaton RougeUSA

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