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Neurochemical Research

, Volume 38, Issue 1, pp 23–31 | Cite as

Endogenous Content and Release of [3H]-GABA and [3H]-Glutamate in the Spinal Cord of Chronically Undernourished Rat

  • Salvador Quiróz-González
  • Rodrigo Erick Escartín-Pérez
  • Francisco Paz-Bermudez
  • Bertha Segura-Alegría
  • Celia Reyes-Legorreta
  • José Carlos Guadarrama-Olmos
  • Benjamin Florán-Garduño
  • Ismael Jiménez-Estrada
Original Paper

Abstract

The aim of this study was to determine the effect of chronic undernutrition on the content and release of γ-amino butyric acid (GABA) and glutamate (GLU) transmitters in the rat spinal cord. The release of [3H]-GABA and [3H]-GLU was determined by radioactive liquid scintillation techniques, and the concentrations of GABA and GLU in spinal cord preparations from control and undernourished young rats (50–60 days old) were measured by reverse-phase HPLC. The GABA and GLU contents in the lumbar spinal dorsal horn (L6 segment) were significantly lower in undernourished rats relative to control rats (22.2 ± 3.7 and 10.7 ± 1.9 %, respectively; P < 0.05). Spinal cord blocks from undernourished animals also showed lower rates of [3H]-GABA and [3H]-GLU release than controls (27.6 ± 3.5 and 12.8 ± 2.5 %, respectively; P < 0.01). We propose that the decreases in GLU content and release are consistent with a reduced activation of either afferent fibers, spinal glutaminergic neurons, or both. Furthermore, we propose that the decreased content and release of GABA in undernourished animals are related to a depression in pre- and post-synaptic inhibition. In addition, we hypothesize that the reductions in GABA content and release serve as compensatory mechanisms to counterbalance decreases in sensory transmission and GLU content in the spinal cord of the chronically undernourished rat.

Keywords

Neurotransmitters Spinal cord Undernutrition GABA Glutamate 

Notes

Acknowledgments

This work was supported by fellowships granted to I. Jiménez-Estrada and B. Florán Garduño by the Sistema Nacional de Investigadores and to Salvador Quiroz by the Consejo Nacional de Ciencia y Tecnología (203774) México.

Conflict of interest

The authors declare that there is no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Salvador Quiróz-González
    • 1
  • Rodrigo Erick Escartín-Pérez
    • 3
  • Francisco Paz-Bermudez
    • 1
  • Bertha Segura-Alegría
    • 2
  • Celia Reyes-Legorreta
    • 4
  • José Carlos Guadarrama-Olmos
    • 1
  • Benjamin Florán-Garduño
    • 1
  • Ismael Jiménez-Estrada
    • 1
  1. 1.Departamento de Fisiología, Biofísica y NeurocienciasCentro de Investigación y Estudios Avanzados del IPNMexicoMexico
  2. 2.Facultad de Estudios Superiores, FES IztacalaUNAMMexicoMexico
  3. 3.Laboratorio de Neurobiología de la Alimentación, FES IztacalaUNAMMexicoMexico
  4. 4.Instituto Nacional de RehabilitaciónSSAMexicoMexico

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