Cholinergic Precursors Modulate the Expression of Heme Oxigenase-1, p21 During Astroglial Cell Proliferation and Differentiation in Culture
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Heme oxygenase-1 (HO-1) plays a crucial role in oxidative stress processes, apoptosis and cell differentiation. Further, some proteins related to cell cycle including cyclins and p21 are important markers of astrocyte cultures. Aim of investigation was to study the effects of cholinergic precursors (choline, CDP-choline, Acetylcholine and α-Glyceril-Phosphorylcholine) on HO-1 and p21 expression during astroglial cell proliferation and differentiation in primary cultures at 14 and 35 days in vitro (DIV) treated for 24 h with choline metabolites. Our results showed a slight reduction of HO-1 expression (data not statistical significant) in astroglial cell cultures treated with CDP-choline at 14 DIV and 35 DIV. On the contrary, ACh and choline induced a significant increase of HO-1 expression in 14 DIV astrocyte cultures. Surprisingly, choline and ACh dramatically reduced HO-1 expression at 35 DIV. A slight decrease not statistical significant was detectable for α-GPC at 14 DIV and particularly significant at 35 DIV. Data concerning p21 expression, a well known protein inhibiting cell cycle, evidenced a significant increase at 14 and 35 DIV after α-GPC treatment. CDP-choline treatment caused a high increase of p21 expression in 14 DIV astrocyte cultures, but no modification at 35 DIV. Instead, ACh treatment induced a marked increment of p21 expression at 35 DIV. Our data suggest that cholinergic precursors modulate HO-1 and p21 expression during astroglial cell proliferation and differentiation in culture and could be considered a tool to study the induced effects of ischemia and hypoxia diseases in some in vitro models to prevent and reduce its effects after treatment with cholinergic drugs.
KeywordsCholine precursors Astroglial cell cultures HO-1 p-21 Proliferation Differentiation
The authors thank very much the pharmaceutical company’s MDM S.p.A. (MB), Italy (mail: firstname.lastname@example.org), for the financial support given to Prof. Roberto Avola’s research group and particularly to Dr. Roberto Gabriele.
Conflict of interest
The authors declare that they have no conflict of interest.
- 2.Carnevale D, De Simone R, Minghetti L (2007) Microglia-neuron interaction in inflammatory and degenerative diseases: role of cholinergic and noradrenergic systems. CNS Neurol Disord Drug Targets 6:388–397 1871-5273/07. doi: 10.2174/187152707783399193
- 3.Bessis A, Bechade C, Bernard D, Roumier A (2007) Microglia-neuron interaction in inflammatory and degenerative diseases: role of cholinergic and noradrenergic systems. CNS Neurol Disord Drug Targets 6:388–397 1871-5273/07. doi: 10.2174/187152707783399193
- 11.Shibahara S, Yoshizawa M, Suzuki H et al (1993) Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. J Biochem (Tokyo) 113:214–218Google Scholar
- 13.McCoubrey WK Jr, Ewing JF, Maines MD (1992) Human heme oxygenase-2: characterization and expression of a full-length cDNA and evidence suggesting that the two HO-2 transcripts may differ by choice of polyadenylation signal. Arch Biochem Biophys 295:13–20. doi: 10.1016/0003-9861(92)90481-B PubMedCrossRefGoogle Scholar
- 18.Cragnolini A.B, Volosin M, Huang Y et al (2011) Nerve Growth Factor Induces Cell Cycle Arrest of Astrocytes. Dev Neurobiol doi: 10.1002/dneu.20981
- 25.Moon DO, Choi YH, Kim GY (2011) Role of p21 in SP600125-induced cell cycle arrest, endoreduplication and apoptosis. Cell Mol Life Sci 68(19):3249–3260Google Scholar
- 33.Bramanti V, Bronzi D, Tomassoni D, Li Volti G, Cannavò G, Raciti G, Napoli M, Vanella A, Campisi A, Ientile R, Avola R (2008) Effect of choline-containing phospholipids on transglutaminase activity in primary astroglial cell cultures. Clin Exp Hypertens 30(8):798–807. doi: 10.1080/10641960802563576 PubMedCrossRefGoogle Scholar