Neurochemical Research

, Volume 37, Issue 6, pp 1150–1153 | Cite as

On the Structural Elucidation of GalNAc-GD1a

  • Yu-Teh Li
Review Article


This short recollection on the structural elucidation of GalNAc-GD1a [1] is in honor of Prof. Robert Ledeen’s lifetime achievements and his immeasurable contributions to advance the field of neurochemistry. This brief historical account also serves as an opportunity to pay my personal tribute to the late Prof. Saul Roseman and the late Prof. Lars Svennerholm, two legendary giants who shaped the field of glycobiology. With nostalgia I am reminiscing with a personal account of my unexpected chance encounter with Svennerholm at the Johns Hopkins University through Roseman in 1971. It is with deep regret that I did not have the opportunity to tell this story to Roseman in person before his passing on July 2, 2011.

Together with Su-Chen (my wife), I have devoted my entire scientific career to study the structure, function and pathobiology of glycoconjugates, and my serendipitous meeting with Svennerholm stemmed from our structural studies of the glycosphingolipid (GSL),...


Sialic Acid GlcNAc Glycopeptide Sugar Chain Lysosomal Storage Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



My special thanks goes to Su-Chen Li for her never-failing support. I would also like to thank Gilbert Estrada for his invaluable input and proofreading of the manuscript.


  1. 1.
    Svennerholm L, Mansson JE, Li YT (1973) Isolation and structural determination of a novel ganglioside, a disialosylpentahexosylceramide from human brain. J Biol Chem 248:740–742PubMedGoogle Scholar
  2. 2.
    Sweeley CC, Klionsky B (1963) Fabry’s disease: classification as a sphingolipidosis and partial characterization of a novel glycolipid. J Biol Chem 238:3148–3150PubMedGoogle Scholar
  3. 3.
    Sweeley CC, Snyder PD Jr, Griffin CE (1970) Chemistry of glycosphingolipids in Fabry’s disease. Chem Phys Lipids 4:393–408PubMedCrossRefGoogle Scholar
  4. 4.
    Sweeley CC (2010) Reflections on my career in analytical chemistry and biochemistry. Proc Jpn Acad Ser B Phys Biol Sci 86:822–836PubMedCrossRefGoogle Scholar
  5. 5.
    Li SC, Mazzotta MY, Chien SF, Li YT (1975) Isolation and characterization of jack bean beta-galactosidase. J Biol Chem 250:6786–6791PubMedGoogle Scholar
  6. 6.
    Li YT, Li SC (1972) Alpha-galactosidase from figs. Methods Enzymol 28:714–720CrossRefGoogle Scholar
  7. 7.
    Li YT, Li SC (1971) Anomeric configuration of galactose residues in ceramide trihexosides. J Biol Chem 246:3769–3771PubMedGoogle Scholar
  8. 8.
    Li YT, Li SC, Shetlar MR (1968) Isolation of glycopeptides from rat liver microsomes involved in the biosynthesis of plasma glycoprotein. J Biol Chem 243:656–665PubMedGoogle Scholar
  9. 9.
    Lee YC (1971) A common structural feature of asparagine-oligosaccharides from glycoproteins. Fed Proc 30:1223Google Scholar
  10. 10.
    Li YT, Lee YC (1972) Pineapple alpha- and beta-D-mannopyranosidases and their action on core glycopeptides. J Biol Chem 247:3677–3683PubMedGoogle Scholar
  11. 11.
    Comb DG, Roseman S (1958) Composition and enzymatic synthesis of N-acetylneuraminic acid (sialic acid). J Am Chem Soc 80:497–499CrossRefGoogle Scholar
  12. 12.
    Comb DG, Roseman S (1960) The sialic acids. I. The structure and enzymatic synthesis of N-acetylneuraminic acid. J Biol Chem 235:2529–2537PubMedGoogle Scholar
  13. 13.
    Blix G, Svennerholm L, Werner I (1952) The isolation of chondrosamine from gangliosides and from submaxillary mucin. Acta Chem Scand 6:358–362CrossRefGoogle Scholar
  14. 14.
    Svennerholm L (1963) Chromatographic separation of human brain gangliosides. J Neurochem 10:613–623PubMedCrossRefGoogle Scholar
  15. 15.
    Svennerholm L (1964) The gangliosides. J Lipid Res 5:145–155PubMedGoogle Scholar
  16. 16.
    Ledeen R (1970) New developments in the study of ganglioside structures. Chem Phys Lipids 5:205–219PubMedCrossRefGoogle Scholar
  17. 17.
    Hakomori S (1964) A rapid permethylation of glycolipid, and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J Biochem 55:205–208PubMedGoogle Scholar
  18. 18.
    Uncini A, Yuki N (2009) Electrophysiologic and immunopathologic correlates in Guillain–Barre syndrome subtypes. Expert Rev Neurother 9:869–884PubMedCrossRefGoogle Scholar
  19. 19.
    Fujikawa K, Nakashima S, Konishi M, Fuse T, Komura N, Ando T, Ando H, Yuki N, Ishida H, Kiso M (2011) The first total synthesis of ganglioside GalNAc-GD1a, a target molecule for autoantibodies in Guillain–Barre syndrome. Chem Eur J 17:5641–5651PubMedCrossRefGoogle Scholar
  20. 20.
    Li YT, Mazzotta MY, Wan CC, Orth R, Li SC (1973) Hydrolysis of Tay-Sachs ganglioside by beta-hexosaminidase a of human liver and urine. J Biol Chem 248:7512–7515PubMedGoogle Scholar
  21. 21.
    Li SC, Hirabayashi Y, Li YT (1981) A new variant of type-AB GM2-gangliosidosis. Biochem Biophys Res Commun 101:479–485PubMedCrossRefGoogle Scholar
  22. 22.
    Li YT, Maskos K, Chou CW, Cole RB, Li SC (2003) Presence of an unusual GM2 derivative, taurine-conjugated GM2, in Tay-Sachs brain. J Biol Chem 278:35286–35291PubMedCrossRefGoogle Scholar
  23. 23.
    Conzelmann E, Sandhoff K (1978) AB variant of infantile GM2 gangliosidosis: deficiency of a factor necessary for stimulation of hexosaminidase A-catalyzed degradation of ganglioside GM2 and glycolipid GA2. Proc Natl Acad Sci USA 75:3979–3983PubMedCrossRefGoogle Scholar
  24. 24.
    Hirabayashi Y, Li YT, Li SC (1983) The protein activator specific for the enzymic hydrolysis of GM2 ganglioside in normal human brain and brains of three types of GM2 gangliosidosis. J Neurochem 40:168–175PubMedCrossRefGoogle Scholar
  25. 25.
    Gravel RA, Kaback MM, Proia RL, Sandhoff K, Suzuki K, Suzuki K (2001) The GM2 gangliosidoses. In: Scriver CR, Beaudet AL, Sly WS, Valle D, Childs B, Kinzler KW, Vogelstein B (eds) The metabolic and molecular bases of inherited disease. McGraw-Hill, New York, p 3327Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular BiologyTulane University School of MedicineNew OrleansUSA

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