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Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Striatum After Various Durations of Transient Cerebral Ischemia in Gerbils

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Abstract

Ischemic damage occurs well in vulnerable regions of the brain, including the hippocampus and striatum. In the present study, we examined neuronal damage/death and glial changes in the striatum 4 days after 5, 10, 15 and 20 min of transient cerebral ischemia using the gerbil. Spontaneous motor activity was increased with the duration time of ischemia–reperfusion (I-R). To examine neuronal damage, we used Fluoro-Jade B (F-J B, a marker for neuronal degeneration) histofluorescence staining. F-J B positive cells were detected only in the 20 min ischemia-group, not in the other groups. In addition, we examined gliosis of astrocytes and microglia using anti-glial fibrillary acidic protein (GFAP) and anti- ionized calcium-binding adapter molecule 1 (Iba-1), respectively. In the 5 min ischemia-group, GFAP-immunoreactive astrocytes were distinctively increased in number, and the immunoreactivity was stronger than that in the sham-group. In the 10, 15 and 20 min ischemia-groups, GFAP-immunoreactivity was more increased with the duration of I-R. On the other hand, the immunoreactivity and the number of Iba-1-immunoreactive microglia were distinctively increased in the 5 and 10 min ischemia-groups. In the 15 min ischemia-group, cell bodies of microglia were largest, and the immunoreactivity was highest; however, in the 20 min ischemia-group, the immunoreactivity was low compared to the 15 min ischemia-group. The results of western blotting for GFAP and Iba-1 were similar to the immunohistochemical data. In brief, these findings showed that neuronal death could be detected only in the 20 min ischemia-group 4 days after I-R, and the change pattern of astrocytes and microglia were apparently different according to the duration time of I-R.

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Acknowledgments

The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee and Ms. Hyun Sook Kim for their technical help in this study. This work was supported by this work was supported by Rural Development Administration of Agenda project (PJ008261), Korea, and by 2011 Research Grant from Kangwon National University.

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Authors and Affiliations

  1. Department of Emergency Medicine, Hallym University Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, 431-070, South Korea

    Taek Geun Ohk, Seung Min Park & Hee Cheol Ahn

  2. Department of Oral Anatomy, College of Dentistry, Gangneung-Wonju National University, Gangneung, 210-702, South Korea

    Ki-Yeon Yoo

  3. Department of Neurobiology, and Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Bich Na Shin, In Hye Kim, Joon Ha Park & Moo-Ho Won

  4. Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University, Seoul, 140-743, South Korea

    Young Joo Lee

  5. Oriental Bio-herb Research Institute, Kangwon National University, Chuncheon, 200-701, South Korea

    Myong Jo Kim

  6. Fermentation & Food Processing, Department of Korean Food Research for Globalization, NAAS, RDA, Suwon, 441-707, South Korea

    Tae Young Kim

  7. Department of Emergency Medicine, and Institute of Medical Sciences, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Jun Hwi Cho

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  1. Taek Geun Ohk
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Corresponding authors

Correspondence to Moo-Ho Won or Jun Hwi Cho.

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Taek Geun Ohk and Ki-Yeon Yoo contributed equally to this article.

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Ohk, T.G., Yoo, KY., Park, S.M. et al. Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Striatum After Various Durations of Transient Cerebral Ischemia in Gerbils. Neurochem Res 37, 826–834 (2012). https://doi.org/10.1007/s11064-011-0678-9

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  • DOI: https://doi.org/10.1007/s11064-011-0678-9

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