Neurochemical Research

, Volume 37, Issue 3, pp 516–526 | Cite as

Neuroprotective Effect of Sesame Seed Oil in 6-Hydroxydopamine Induced Neurotoxicity in Mice Model: Cellular, Biochemical and Neurochemical Evidence

  • Saif Ahmad
  • M. Badruzzaman Khan
  • M. Nasrul Hoda
  • Kanchan Bhatia
  • Rizwanul Haque
  • Inayat Saleem Fazili
  • Arshad Jamal
  • Jafar Salamt Khan
  • Deepshikha Pande Katare
Original Paper


Natural antioxidants have shown a remarkable reduction in oxidative stress due to excess formation of reactive oxygen species by enhancing antioxidant mechanism in the neurodegenerative disorders. Sesame seed oil (SO) is one of the most important edible oil in India as well as in Asian countries and has potent antioxidant properties thus the present study evaluated the neuroprotective effect of SO by using 6-Hydroxydopamine (6-OHDA)-induced Parkinson’s disease model in mice. The mice were fed an SO mix diet for 15 days and then 6-OHDA was injected into the right striatum of mice brain. Three weeks after 6-OHDA infusion, mice were sacrificed and the striatum was removed. The neuroprotective role of SO on the activities of antioxidant and non-antioxidant enzymes such as glutathione reductase (GR), glutathione-S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT) and content of glutathione (GSH) and thiobarbituric acid reactive substance (TBARS) were studied in the striatum. The activities of all the above-mentioned enzymes decreased significantly in 6-OHDA group (Lesioned) when compared with Sham. The pretreatment of SO on antioxidant mechanism and dopamine level in the brain had shown some significant improvement in Lesion+SO (L+SO) group when compared with Lesioned group. However, NADPH oxidase subunit, Nox2 and inflammatory stimulator Cox2 expression was increased as well as antioxidant MnSOD level was decreased in Lesioned group while SO showed the inhibitory effect on the activation of Nox2 and Cox2 and restored MnSOD expression in L+SO group. Increased tyrosine hydroxylase (TH) expression in substantia nigra as well as dopamine and its metabolite DOPAC level in L+SO group also support our findings that SO may inhibit activation of NADPH oxidase dependent inflammatory mechanism due to 6-OHDA induced neurotoxicity in mice.


Sesame seed oil (SO) Parkinson disease (PD) Neurotoxicity NADPH oxidase Antioxidants Tyrosine hydroxylase 



Authors are thankful to CSIR, Ministry of Science and Technology, New Delhi, Govt. of India for providing authentic sesame seed as a gift sample to carry out this research work. This work was supported by women young scientist award (Department of Science and Technology (DST), New Delhi, Govt. of India) to DPK. We greatly acknowledge Ms Sylvia Megyardi, Oral Biology and Anatomy, GHSU, Augusta, GA, USA for reviewing and editing this manuscript.

Conflict of interest

There is no conflict of interest with any person or other organization.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Saif Ahmad
    • 1
    • 3
  • M. Badruzzaman Khan
    • 2
    • 4
  • M. Nasrul Hoda
    • 2
    • 11
  • Kanchan Bhatia
    • 2
    • 5
  • Rizwanul Haque
    • 2
    • 6
  • Inayat Saleem Fazili
    • 1
    • 7
  • Arshad Jamal
    • 1
    • 8
  • Jafar Salamt Khan
    • 9
  • Deepshikha Pande Katare
    • 1
    • 10
  1. 1.Department of BiotechnologyJamia Hamdard (Hamdard University)New DelhiIndia
  2. 2.Department of Medical Elementology and Toxicology, Faculty of ScienceJamia Hamdard (Hamdard University)New DelhiIndia
  3. 3.Department of Ophthalmology, School of MedicineGeorgia Health Sciences University (GHSU)AugustaUSA
  4. 4.Center for Molecular Chaperone/Radiobiology and Cancer Virology, School of MedicineGeorgia Health Sciences University (GHSU)AugustaUSA
  5. 5.Experimental Medicine, School of MedicineGeorgia Health Sciences University (GHSU)AugustaUSA
  6. 6.Department of Microbiology and ImmunologyC6749 Penn State College of MedicineHersheyUSA
  7. 7.Center of Animal BiotechnologySher-i-Kashmir University of Agricultural Sciences and TechnologySrinagarIndia
  8. 8.Biological Science Department, College of Science and Arts RabighKing Abdulaziz UniversityJeddahSaudi Arabia
  9. 9.Council of Scientific and Industrial Research(CSIR)New DelhiIndia
  10. 10.Amity Institute of BiotechnologyAmity UniversityNoidaIndia
  11. 11.Department of Neurology, School of MedicineGeorgia Health Sciences University (GHSU)AugustaUSA

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