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Involvement of CLEC16A in Activation of Astrocytes After LPS Treated

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Abstract

CLEC16A, C-type lectin domain family 16, member A was recently found to be associated with inflation process in the autoimmune diseases. In this study, we elucidated the dynamic expression changes and localization of CLEC16A in lipopolysaccharide (LPS)-induced neuroinflammatory processes in adult rats. CLEC16A expression was strongly induced in active astrocytes in inflamed cerebral cortex. In vitro studies indicated that the up-regulation of CLEC16A may be involved in the subsequent astrocyte activation following LPS challenge. And Knock-down of CLEC16A in cultured primary astrocytes by siRNA showed that CLEC16A was required for the activation of astrocytes induced by LPS. Collectively, these results suggested CLEC16A may be important in host defense in astrocyte-mediated immune response. Understanding the cell signal pathway may provide a novel strategy against inflammatory and immune reaction in neuroinflammtion in CNS.

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Abbreviations

CLEC16A:

C-type lectin domain family 16, member A

TNF-α:

Tumor necrosis factor-alpha

LPS:

Lipopolysaccharide.

CNS:

Central nervous system

PAGE:

Polyacrylamide gel electrophoresis

ECL:

Enhanced chemiluminescence system

RT-PCR:

Reverse transcriptase PCR

GFAP:

Glial fibrillary acidic protein

NeuN:

Neuronal nuclei

PCNA:

Proliferating cell nuclear antigen

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81070992) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Xinmin Wu, Jing Li, Yaohua Yan, Xiaohong Wu, Bai Shao, Jin Xu & Yilu Gao

  2. Department of Immunology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Jing Li, Chun Chen, Shengyang Jiang, Lihua Kang, Yeqing Huang, Lin Zhu & Yuhong Ji

Authors
  1. Xinmin Wu
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  2. Jing Li
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  3. Chun Chen
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  11. Lin Zhu
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  13. Yilu Gao
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Corresponding authors

Correspondence to Yuhong Ji or Yilu Gao.

Additional information

Yilu Gao is the first corresponding author.

Xinmin Wu and Jing Li contributed equally to this work.

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Wu, X., Li, J., Chen, C. et al. Involvement of CLEC16A in Activation of Astrocytes After LPS Treated. Neurochem Res 37, 5–14 (2012). https://doi.org/10.1007/s11064-011-0581-4

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  • DOI: https://doi.org/10.1007/s11064-011-0581-4

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