Neurochemical Research

, 36:2427 | Cite as

Retinal Metabolic Changes in an Experimental Model of Optic Nerve Transection by Ex Vivo 1H Magnetic Resonance Spectroscopy

  • Shuang Li
  • Mingming Huang
  • Xinghua Wang
  • Xuxia Wang
  • Fei Chen
  • Hao Lei
  • Fagang Jiang
Original Paper


This study aims to investigate the retinal metabolic processes in a rat axotomy model. Retinal metabolic changes in optic nerve transection (ONT) rat model were analyzed by 1H magnetic resonance spectroscopy (1H-MRS). Retinal ganglion cells (RGCs) densities were assessed from retinal whole mounts. The retina was stained immunohistochemically with glial fibrillary acidic protein (GFAP). The results showed that the retina in ONT rats had significantly decreased concentrations of γ-aminobutyric acid (GABA), N-acetylaspartate (NAA), taurine (Tau), creatine (Cr) and increased concentrations of alanine (Ala) compared with control. Examination of glutamate (Glu), glutamine (Gln) and Glx (Glu + Gln) concentrations disclosed no significant differences. The mean density of RGCs reduced from 2,249 ± 87 cells/mm2 in control group to 320 ± 56 cells/mm2 in ONT group. GFAP immunoreactivity was markedly higher in ONT group than that in control group. The retinal metabolism after ONT was associated with neurotransmitter recycling/production perturbation, as well as other metabolic disequilibrium.


Optic nerve transection Glutamate GABA Magnetic resonance spectroscopy Metabolism 



This work was supported by grant 30700922 from National Natural Science Foundation of China.

Conflicts of interest

No potential conflicts of interest are disclosed.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Shuang Li
    • 1
  • Mingming Huang
    • 2
  • Xinghua Wang
    • 1
  • Xuxia Wang
    • 2
  • Fei Chen
    • 1
  • Hao Lei
    • 2
  • Fagang Jiang
    • 1
  1. 1.Department of Ophthalmology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhanChina

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