Neurochemical Research

, Volume 36, Issue 10, pp 1931–1938 | Cite as

Age-Related Binding of Proximal Region of ApoE Promoter to Nuclear Proteins of Mouse Cerebral Cortex

  • Sarika Singh
  • M. K. Thakur
Original Paper


Mouse ApolipoproteinE (ApoE) gene has maximum promoter activity in the proximal region (−212 to +54) which includes different regulatory elements. These elements bind to specific protein factors and influence the expression of genes which are involved in key brain functions that decline with age. As there is no information on the binding of apoE promoter to nuclear proteins as a function of age, we have analyzed the binding of USF, AP1 and one negative element sequence present in ApoE proximal promoter to nuclear proteins of the cerebral cortex of mice of different ages. The findings show the formation of one complex with USF and two complexes with AP1 and negative element. The intensity of these complexes varies with age, indicating differential binding of protein factors to specific elements of apoE promoter, which reflect age-related regulation of apoE -mediated brain functions.


ApolipoproteinE. Aging Alzheimer’s disease EMSA Transcription factor USF AP1 



This work was supported by grants from the Indian Council of Medical Research (No53/6/2004-BMS), Government of India, to MKT. Sarika is a recipient of senior research fellowship from the Council of Scientific and Industrial Research, India.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Biochemistry and Molecular Biology Laboratory, Department of ZoologyBanaras Hindu UniversityVaranasiIndia

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