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Presence of Phosphatidylserine Synthesizing Enzymes in Triton Insoluble Floating Fractions from Cerebrocortical Plasma Membranes

Do Phosphatidylserine Synthesizing Enzymes in Plasma Membrane Microdomains Play a Role in Signal Transduction?

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Abstract

Mammals synthesize phosphatidylserine (PS), a binding PKC molecule, by exchanging the nitrogen base of phosphatidylethanolamine or phosphatidylcholine with free serine. Serine base exchange enzyme (SBEE) was found in Triton insoluble floating fractions (TIFFs) from rat cerebellum which contained PKC. Consequently, SBEE might modulate PS levels in the PKC binding area (Buratta et al., J Neurochem 103:942–951, 2007). In the present study, we determined whether SBEE and PKC were localised in rat cerebral cortex TIFFs (cx-TIFFs) and in rat cerebrocortical plasma membrane-TIFFs (PM-TIFFs) which are more directly involved in signal transduction than intracellular membranes. Cx-and PM-TIFFs expressed SBEE activity and contained PKC. SBEE used ethanolamine as free exchanging base which may modulate PS level in the PKC binding area, transforming PS into PE and vice versa. The slight decrease in [14C]serine incorporation in the presence of choline indicated the existence of a SBEE isoform which may play a peculiar role in this brain area.

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Acknowledgments

The authors would like to thank Dr G.A. Boyd for English editing of this paper. This work was supported by the Italian Ministry of University Education and Research (MIUR).

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  1. Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06127, Perugia, Italy

    Sandra Buratta, Giuseppina Ferrara & Rita Mozzi

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  1. Sandra Buratta
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  2. Giuseppina Ferrara
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  3. Rita Mozzi
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Correspondence to Rita Mozzi.

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Buratta, S., Ferrara, G. & Mozzi, R. Presence of Phosphatidylserine Synthesizing Enzymes in Triton Insoluble Floating Fractions from Cerebrocortical Plasma Membranes. Neurochem Res 36, 774–782 (2011). https://doi.org/10.1007/s11064-011-0399-0

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  • DOI: https://doi.org/10.1007/s11064-011-0399-0

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