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Acute Hypoxia Differentially Affects the NMDA Receptor NR1, NR2A and NR2B Subunit mRNA Levels in the Developing Chick Optic Tectum: Stage-Dependent Plasticity in the 2B–2A Ratio

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Abstract

It is known that the NMDA-R NR1 subunit is needed for the receptor activity and that under hypoxia the evolution toward apoptosis or neuronal survival depends on the balance NR2A/NR2B subunits. This paper analyzes the effect of acute hypoxia on the above mentioned subunits mRNAs during development. The mean percentage of NR1+ neurons displayed the higher plasticity during development while the NR2A+ neurons the higher stability. Acute hypoxia increased the mean percentage of NR1+ and NR2B+ neurons at ED12 but only that of NR1+ neurons at ED18. Acute hypoxia increased the levels of expression of NR1 and NR2B mRNAs at ED12 without changes in the NR2A mRNA. During early stages there is a higher sensitivity to change the subunits mRNA levels under a hypoxic treatment. At ED12 acute hypoxia increased the probability of co-expression of the NR1–NR2A and NR1–NR2B subunits combinations, the level of NR1 and NR2B and the ratio NR2B/NR2A. These conditions facilitate the evolution towards apoptosis.

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Acknowledgments

This work was supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, PIP 5410) and Agencia Nacional para la Promoción Científica y Tecnológica (FONCYT, PICT 38234).

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Authors and Affiliations

  1. Institute of Cell Biology and Neuroscience “Prof. E. De Robertis”, School of Medicine, University of Buenos Aires, Paraguay 2155, 1121, Buenos Aires, Argentina

    Marina Vacotto, Vladimir Flores & Sara Fiszer de Plazas

  2. Department of Biostructural Sciences, Interdisciplinary Group in Theoretical Biology, Favaloro University, Buenos Aires, Argentina

    Melina Rapacioli & Vladimir Flores

Authors
  1. Marina Vacotto
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  2. Melina Rapacioli
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  3. Vladimir Flores
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  4. Sara Fiszer de Plazas
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Correspondence to Sara Fiszer de Plazas.

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Vacotto, M., Rapacioli, M., Flores, V. et al. Acute Hypoxia Differentially Affects the NMDA Receptor NR1, NR2A and NR2B Subunit mRNA Levels in the Developing Chick Optic Tectum: Stage-Dependent Plasticity in the 2B–2A Ratio. Neurochem Res 35, 1609–1619 (2010). https://doi.org/10.1007/s11064-010-0221-4

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