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Recovery of Motor Deficit, Cerebellar Serotonin and Lipid Peroxidation Levels in the Cortex of Injured Rats

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Abstract

The sensorimotor cortex and the cerebellum are interconnected by the corticopontocerebellar (CPC) pathway and by neuronal groups such as the serotonergic system. Our aims were to determine the levels of cerebellar serotonin (5-HT) and lipid peroxidation (LP) after cortical iron injection and to analyze the motor function produced by the injury. Rats were divided into the following three groups: control, injured and recovering. Motor function was evaluated using the beam-walking test as an assessment of overall locomotor function and the footprint test as an assessment of gait. We also determined the levels of 5-HT and LP two and twenty days post-lesion. We found an increase in cerebellar 5-HT and a concomitant increase in LP in the pons and cerebellum of injured rats, which correlated with their motor deficits. Recovering rats showed normal 5-HT and LP levels. The increase of 5-HT in injured rats could be a result of serotonergic axonal injury after cortical iron injection. The LP and motor deficits could be due to impairments in neuronal connectivity affecting the corticospinal and CPC tracts and dysmetric stride could be indicative of an ataxic gait that involves the cerebellum.

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Authors and Affiliations

  1. Depto de Neurofisiologia-Lab. de Cromatografia y Microdialisis, INR, SSA, Calz. Mexico-Xochimilco 289 Col. Arenal de Guadalupe Deleg. Tlalpan, C.P. 42389, Mexico City, Mexico

    Antonio Bueno-Nava

  2. Depto de Neurofisiologia-Lab. de Neuroplasticidad, Torre de Investigación, Instituto Nacional de Rehabilitación, SSA, Calz. México-Xochimilco 289, Col. Arenal de Guadalupe, Deleg. Tlalpan, C.P. 14389, Mexico City, Mexico

    Rigoberto Gonzalez-Pina

  3. Depto de Neurofisiologia-Lab. de Neuroquimica, INR, SSA, Mexico City, Mexico

    Alfonso Alfaro-Rodriguez

  4. Depto de Neurofisiologia-Lab. de Neuroprotección, INR, SSA, Mexico City, Mexico

    Vladimir Nekrassov-Protasova & Alfredo Durand-Rivera

  5. Depto de Neuroquimica, INNN MVS, SSA, Mexico City, Mexico

    Sergio Montes

  6. Facultad de Psicologia, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico

    Fructuoso Ayala-Guerrero

Authors
  1. Antonio Bueno-Nava
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  2. Rigoberto Gonzalez-Pina
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  3. Alfonso Alfaro-Rodriguez
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  4. Vladimir Nekrassov-Protasova
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  5. Alfredo Durand-Rivera
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  6. Sergio Montes
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  7. Fructuoso Ayala-Guerrero
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Correspondence to Rigoberto Gonzalez-Pina.

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Bueno-Nava, A., Gonzalez-Pina, R., Alfaro-Rodriguez, A. et al. Recovery of Motor Deficit, Cerebellar Serotonin and Lipid Peroxidation Levels in the Cortex of Injured Rats. Neurochem Res 35, 1538–1545 (2010). https://doi.org/10.1007/s11064-010-0213-4

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