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Neurochemical Research

, Volume 35, Issue 9, pp 1455–1470 | Cite as

Characterization of Neural Stem/Progenitor Cells Expressing VEGF and its Receptors in the Subventricular Zone of Newborn Piglet Brain

  • Jahan Ara
  • Saskia Fekete
  • Anli Zhu
  • Melissa Frank
ORIGINAL PAPER

Abstract

Neural stem/progenitor cell (NSP) biology and neurogenesis in adult central nervous system (CNS) are important both towards potential future therapeutic applications for CNS repair, and for the fundamental function of the CNS. In the present study, we report the characterization of NSP population from subventricular zone (SVZ) of neonatal piglet brain using in vivo and in vitro systems. We show that the nestin and vimentin-positive neural progenitor cells are present in the SVZ of the lateral ventricles of neonatal piglet brain. In vitro, piglet NSPs proliferated as neurospheres, expressed the typical protein of neural progenitors, nestin and a range of well-established neurodevelopmental markers. Upon dissociation and subculture, piglet NSPs differentiated into neurons and glial cells. Clonal analysis demonstrates that piglet NSPs are multipotent and retain the capacity to generate both glia and neurons. These cells expressed VEGF, VEGFR1, VEGFR2 and Neuropilin-1 and -2 mRNAs. Real time PCR revealed that SVZ NSPs from newborn piglet expressed total VEGF and all VEGF splice variants. These findings show that piglet NSPs may be helpful to more effectively design growth factor based strategies to enhance endogenous precursor cells for cell transplantation studies potentially leading to the application of this strategy in the nervous system disease and injury.

Keywords

Neural stem/progenitor cells Subventricular zone Proliferation Newborn brain Piglet VEGF 

Notes

Acknowledgments

We thank Dr. Judy Grinspan, Children’s Hospital of Philadelphia and University of Pennsylvania, for kindly providing the anti-O4 and Anti-GFAP antibodies. We also thank Dr. David Pleasure, UC Davis School of Medicine/Shriners Hospital, California, for critically reviewing the manuscript. This work was supported by American Heart Association #0835233 N; March of Dimes Foundation #6-FY09-321 and Saint Christopher’s Foundation for Children 270675 (to JA).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Jahan Ara
    • 1
  • Saskia Fekete
    • 2
  • Anli Zhu
    • 1
  • Melissa Frank
    • 1
  1. 1.Department of PediatricsDrexel University College of Medicine and Saint Christopher’s Hospital for ChildrenPhiladelphiaUSA
  2. 2.Faculdade de Medicina de BotucatuUniversidade Estadual PaulistaBotucatuBrazil

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