Neurochemical Research

, Volume 35, Issue 5, pp 727–734 | Cite as

Neurturin Effects on Nigrostriatal Dopamine Release and Content: Comparison with GDNF

  • Wayne A. Cass
  • Laura E. Peters
Original Paper


Neurturin (NTN) is a member of the glial cell line-derived neurotrophic factor (GDNF) family; and, while GDNF has been shown to increase dopamine (DA) release in normal animals, the ability of NTN to alter DA release has not been previously reported. The purpose of the present study was to determine if NTN could alter striatal DA release, and to compare the effects of NTN to GDNF. Male Fischer-344 rats were given a single injection of vehicle or 5 μg NTN or GDNF into the right substantia nigra. Three weeks later microdialysis experiments were conducted to assess striatal DA release. Basal extracellular levels of striatal DA were not affected by either NTN or GDNF. However, both NTN and GDNF led to increases in amphetamine-evoked overflow of DA from the ipsilateral striatum, and there was a trend for potassium-evoked overflow to be augmented. Postmortem tissue levels of DA were decreased by approximately 20% in the striatum, and increased by approximately 100% in the substantia nigra, on the ipsilateral side of the brain compared to the contralateral side following both NTN and GDNF injection. Thus, NTN, like GDNF, can augment striatal DA release, and the magnitude of the NTN effects are similar to those of GDNF.


Neurturin GDNF Striatum Substantia nigra Dopamine Microdialysis 



This study was supported in part by United States Public Health Service Grant AG17963.


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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Anatomy and Neurobiology, MN-225 Chandler Medical CenterUniversity of Kentucky College of MedicineLexingtonUSA

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