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Neurochemical Research

, 34:1907 | Cite as

The Potential Role of Nitric Oxide Synthase in Survival and Regeneration of Magnocellular Neurons of Hypothalamo-Neurohypophyseal System

  • Qiuju Yuan
  • David E. Scott
  • Kwow-Fai So
  • Zhixiu Lin
  • Wutian Wu
Original Paper

Abstract

Previous investigations from this laboratory have demonstrated that hypophysectomy induces up-regulation of neuronal nitric oxide synthase (nNOS) in magnocellular neurons of the mammalian hypothalamo-neurohypophyseal system (HNS). Accompanied by this upregulation of nNOS, both neuronal regeneration and degeneration are also observed in this system following hypophysectomy. The specific aim of this study was to determine the potential role of nNOS upregulation in neuronal survival and regeneration after hypophysectomy in the adult Sprague–Dawley (SD) rat by using a competitive nitric oxide synthase blocker, N(G)-nitrol-l-arginine methyl ester (l-NAME). We found that l-NAME treatment effectively blocked the regeneration of magnocellular neurons of the rodent hypothalamus as observed in the lumen of the third cerebral ventricle following hypophysectomy. However, l-NAME had no effect on the survival of magnocellular neurons in the supraoptic (SON) and paraventricular (PVN) nuclei after hypophysectomy. These results suggest that the induced increase of nNOS expression enhance the regenerative ability of magnocellular neurons of the HNS following hypophysectomy.

Keywords

Neuronal plasticity Nitric oxide Axonal injury Endocrine hypothalamus 

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Qiuju Yuan
    • 1
    • 4
  • David E. Scott
    • 5
  • Kwow-Fai So
    • 1
    • 2
  • Zhixiu Lin
    • 4
  • Wutian Wu
    • 1
    • 2
    • 3
  1. 1.Department of Anatomy, Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamChina
  2. 2.State Key Laboratory of Brain and Cognitive Sciences, Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamChina
  3. 3.Research Center of Reproduction, Development and Growth, Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamChina
  4. 4.School of Chinese Medicine, Faculty of ScienceThe Chinese University of Hong KongShatin, N.TChina
  5. 5.The Department of Pathology & AnatomyThe Eastern Virginia Medical SchoolNorfolkUSA

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