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Neuroprotective Effect of DAHK Peptide in an Occlusive Model of Permanent Focal Ischemia in Rats

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Abstract

This study examined the neuroprotective ability of tetrapeptide l-Asp-Ala-His-Lys (DAHK) in permanent middle cerebral artery occlusion in rats. One DAHK dose (16 mg/kg) or saline solution were i.v. administered 30 min after occlusion and neurological deficit was evaluated at 2, 24, 48, 72 and 96 h using Longa scoring scale. The striatum infarction area was evaluated until 96 h after occlusion in both groups after staining with hematoxylin–eosin. DAHK-treated group showed a significant (P < 0.05) protection of 70% of neurological deficit at 96 h after occlusion, in comparison with the control-group that showed permanent neurological deficit. The DAHK-treated group showed a significant (P < 0.05) reduction of 52% infarction area in the striatum, as compared to control values. Results presented here support the possible therapeutic application of DAHK as a neuroprotective agent in human patients with stroke, as the peptide is part of human serum albumin, already being tested in clinical trials.

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Acknowledgements

We want to thank the Fundación Miguel Alemán A.C. for the support of this study, and the support through a PROMEP/SEP fellowship granted to J. Carbajal-Sotelo. The authors acknowledged the support received from the laboratory of Dr. Lourival Possani from the Biotechnology Institute of UNAM.

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Authors and Affiliations

  1. Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, “Manuel Velasco Suárez”, Av. Insurgentes Sur No. 3877, C.P. 14269, Mexico City, D.F., Mexico

    Araceli Díaz-Ruíz, Camilo Ríos & Sergio Montes

  2. Laboratorio de Neuroprotección, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa, C.P. 62210, Cuernavaca, Morelos, Mexico

    Joselyn Carvajal-Sotelo & Antonio Monroy-Noyola

  3. Departamento de Neuropatología, Instituto Nacional de Neurología y Neurocirugía, “Manuel Velasco Suárez”, Av. Insurgentes Sur No. 3877, C.P. 14269, Mexico City, D.F., Mexico

    Alma Ortiz-Plata & Marisela Méndez-Armenta

  4. Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001. Col. Chamilpa, C.P. 62210, Cuernavaca, Morelos, Mexico

    Gerardo Pavel Espino-Solis

Authors
  1. Araceli Díaz-Ruíz
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  2. Camilo Ríos
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  3. Joselyn Carvajal-Sotelo
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  4. Alma Ortiz-Plata
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  5. Gerardo Pavel Espino-Solis
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  6. Marisela Méndez-Armenta
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  7. Sergio Montes
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  8. Antonio Monroy-Noyola
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Correspondence to Antonio Monroy-Noyola.

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Díaz-Ruíz, A., Ríos, C., Carvajal-Sotelo, J. et al. Neuroprotective Effect of DAHK Peptide in an Occlusive Model of Permanent Focal Ischemia in Rats. Neurochem Res 35, 343–347 (2010). https://doi.org/10.1007/s11064-009-0060-3

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  • DOI: https://doi.org/10.1007/s11064-009-0060-3

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