Neuroprotective Effect of DAHK Peptide in an Occlusive Model of Permanent Focal Ischemia in Rats
- 118 Downloads
This study examined the neuroprotective ability of tetrapeptide l-Asp-Ala-His-Lys (DAHK) in permanent middle cerebral artery occlusion in rats. One DAHK dose (16 mg/kg) or saline solution were i.v. administered 30 min after occlusion and neurological deficit was evaluated at 2, 24, 48, 72 and 96 h using Longa scoring scale. The striatum infarction area was evaluated until 96 h after occlusion in both groups after staining with hematoxylin–eosin. DAHK-treated group showed a significant (P < 0.05) protection of 70% of neurological deficit at 96 h after occlusion, in comparison with the control-group that showed permanent neurological deficit. The DAHK-treated group showed a significant (P < 0.05) reduction of 52% infarction area in the striatum, as compared to control values. Results presented here support the possible therapeutic application of DAHK as a neuroprotective agent in human patients with stroke, as the peptide is part of human serum albumin, already being tested in clinical trials.
KeywordsIschemia Stroke DAHK peptide Neuroprotection
We want to thank the Fundación Miguel Alemán A.C. for the support of this study, and the support through a PROMEP/SEP fellowship granted to J. Carbajal-Sotelo. The authors acknowledged the support received from the laboratory of Dr. Lourival Possani from the Biotechnology Institute of UNAM.
- 2.American Heart Association (2005) Heart disease and stroke statistics. American Heart Association, DallasGoogle Scholar
- 7.Alonso de Leciñana M, Diez-Tejedor E, Gutierrez M, Guerrero S, Carceller F, Roda JM (2005) New goals in ischemic stroke therapy: the experimental approach-harmonizing science with practice. Cerebrovasc Dis 20:59–168Google Scholar
- 13.Batista CV, Del Pozo L, Zamudio FZ, Contreras S, Becerril B, Wanke E, Possani LD (2004) Proteomics of the venom from the Amazonian scorpion Tityus cambridgei and the role of prolines on mass spectrometry analysis of toxins. J Chromatogr B Analyt Technol Biomed Life Sci 803:55–66CrossRefPubMedGoogle Scholar
- 17.Paxinos G, Watson C (1998) The rat brain in stereotaxic coordinates. Academic Press, San Diego, p 340Google Scholar