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Neurochemical Research

, Volume 35, Issue 2, pp 298–305 | Cite as

Promotion of Lipid and Protein Oxidative Damage in Rat Brain by Ethylmalonic Acid

  • Patrícia Fernanda Schuck
  • Estela Natacha Brandt Busanello
  • Alana Pimentel Moura
  • Anelise Miotti Tonin
  • Mateus Grings
  • Luciana Ritter
  • Carmen Regla Vargas
  • Gustavo da Costa Ferreira
  • Moacir Wajner
Original Paper

Abstract

High concentrations of ethylmalonic acid are found in tissues and biological fluids of patients affected by ethylmalonic encephalopathy, deficiency of short-chain acyl-CoA dehydrogenase activity and other illnesses characterized by developmental delay and neuromuscular symptoms. The pathophysiological mechanisms responsible for the brain damage in these patients are virtually unknown. Therefore, in the present work we investigated the in vitro effect of EMA on oxidative stress parameters in rat cerebral cortex. EMA significantly increased chemiluminescence and thiobarbituric acid-reactive species levels (lipoperoxidation), as well as carbonyl content and oxidation of sulfhydryl groups (protein oxidative damage) and DCFH. EMA also significantly decreased the levels of reduced glutathione (non-enzymatic antioxidant defenses). In contrast, nitrate and nitrite levels were not affected by this short organic acid. It is therefore presumed that oxidative stress may represent a pathomechanism involved in the pathophysiology of the neurologic symptoms manifested by patients affected by disorders in which EMA accumulates.

Keywords

Reactive oxygen species Ethylmalonic acid SCAD deficiency Oxidative stress Rat brain 

Notes

Acknowledgments

This work was supported by grants from CNPq, PRONEX II, FAPERGS, PROPESQ/UFRGS, and FINEP research grant Rede Instituto Brasileiro de Neurociência (IBN-Net) # 01.06.0842-00, Instituto Nacional de Ciência e Tecnologia para Excitotoxicidade e Neuroproteção (INCT-EM).

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Patrícia Fernanda Schuck
    • 1
    • 3
  • Estela Natacha Brandt Busanello
    • 1
  • Alana Pimentel Moura
    • 1
  • Anelise Miotti Tonin
    • 1
  • Mateus Grings
    • 1
  • Luciana Ritter
    • 1
  • Carmen Regla Vargas
    • 2
  • Gustavo da Costa Ferreira
    • 1
  • Moacir Wajner
    • 1
    • 2
  1. 1.Departamento de Bioquímica, Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Serviço de Genética Médica do Hospital de Clínicas de Porto AlegrePorto AlegreBrazil
  3. 3.Laboratório de Fisiopatologia Experimental, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da SaúdeUniversidade do Extremo Sul CatarinenseCriciúmaBrazil

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