Protein Kinase C Regulates the Expression of M1 Receptors and BDNF in Rat Retinal Cells
Protein kinase C (PKC) plays a key role in cellular events including proliferation, survival and differentiation. Our previous study showed the effect of phorbol 12-myristate 13-acetate (PMA), a PKC activator, inducing a decrease in retinal cells proliferation. This effect was mediated by muscarinic type 1 receptors (M1) activation and brain derived neurotrophic factor (BDNF) treatment also induced a decrease in cell proliferation. Based on these results we analyzed the expression of either M1 receptors or BDNF following PMA treatment of retinal cell cultures. Our data demonstrated that PMA induced a decrease in both protein expressions after 48 h in culture. However, after 45 min, PMA induced a transient increase in BDNF expression and a decrease in M1 receptors expression. Analyzing the expression of M1 receptors and BDNF during the postnatal development in vivo, we observed a decrease in both proteins. Taken together our results suggest the involvement of PKC in the control of M1 expression in retinal cells.
KeywordsPMA BDNF M1 receptors Rat retina
We would like to thank Alexandre José Fernandes, Bernardino Matheus dos Santos and Alecsandro de Jesus Rezende for technical assistance and Arnaldo Paes de Andrade for his helpful discussions. Aline Araujo dos Santos was the recipient of a CAPES fellowship and Simone Vidal Medina was the recipient of a FAPERJ fellowship. This work was supported by grants from CAPES, CNPq, PRONEX-MCT, and FAPERJ.
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