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Neurochemical Research

, Volume 33, Issue 12, pp 2575–2582 | Cite as

Renal Expression of the Brain and Muscle Isoforms of Glycogen Phosphorylase in Different Cell Types

  • Heide Schmid
  • Benedikt Dolderer
  • Ulrike Thiess
  • Stephan Verleysdonk
  • Bernd Hamprecht
Original Paper

Abstract

Kidney contains glycogen. Glycogen is degraded by glycogen phosphorylase (GP). This enzyme comes in three isoforms, one of which, the brain isozyme (GP BB), is known to occur in kidney. Its pattern of distribution in rat kidney was studied in comparison to that of the muscle isoform (GP MM) with the aim to see if for GP BB and GP MM there were functional similarities in brain and kidney. In immunoblotting and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) experiments, both isozymes and their respective mRNAs were found in kidney homogenates. GP BB was immunocytochemically detected in collecting ducts which were identified by the marker protein aquaporin-2. GP MM was localized exclusively in interstitial cells of cortex and outer medulla. These cells were identified as fibroblasts by their expression of 5′-ectonucleotidase (cortex) or by their morphology (outer medulla). The physiological role of both isozymes is discussed in respect to local demands of energy and of proteoglycan building blocks.

Keywords

Glycogen phosphorylase Brain isozyme Muscle isozyme Rat kidney Collecting duct Fibroblast 

Notes

Acknowledgement

We wish to gratefully acknowledge Dr. Brigitte Pfeiffer-Guglielmi’s exploratory experiments that stimulated the initiation of the present study. Thanks are also due to Professor Brigitte Kaissling and Dr. Hubert Loewenheim for their generous gifts of antibodies and to Barbara Birk for technical advice.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Heide Schmid
    • 1
  • Benedikt Dolderer
    • 1
  • Ulrike Thiess
    • 1
  • Stephan Verleysdonk
    • 1
  • Bernd Hamprecht
    • 1
  1. 1.Interfaculty Institute for BiochemistryUniversity of TuebingenTuebingenGermany

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