Neurochemical Research

, Volume 33, Issue 7, pp 1341–1345 | Cite as

Novel Form of Phosphate Activated Glutaminase in Cultured Astrocytes and Human Neuroblastoma Cells, PAG in Brain Pathology and Localization in the Mitochondria

  • E. Kvamme
  • L. S. H. Nissen-Meyer
  • B. Å. Roberg
  • I. Aa. Torgner
Original Paper


A novel form of phosphate activated glutaminase (PAG), catalyzing the synthesis of glutamate from glutamine, has been detected in cultured astrocytes and SH-SY5Y neuroblastoma cells. This enzyme form is different from that of the kidney and liver isozymes. In these cells we found high enzyme activity, but no or very weak immunoreactivity against the kidney type of PAG, and no immunoreactivity against the liver type. PAG was also investigated in brain under pathological conditions. In patients with Down’s syndrome the immunoreactivity in the frontoparietal cortex was significantly reduced. The findings leading to our conclusion of a functionally active PAG on the outer face of the inner mitochondrial membrane are discussed, and a model is presented.


Phosphate activated glutaminase Brain glutaminase Submitochondrial localization Astrocytes Neuroblastoma cells Brain pathology 


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • E. Kvamme
    • 1
  • L. S. H. Nissen-Meyer
    • 2
  • B. Å. Roberg
    • 1
  • I. Aa. Torgner
    • 1
  1. 1.Neurochemical Section, Department of BiochemistryInstitute of Basic Medical Sciences, University of OsloOsloNorway
  2. 2.The Biotechnology Centre of OsloUniversity of OsloOsloNorway

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