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Dex-ras1 and Serum- and Glucocorticoid-inducible Protein Kinase 1: Regulation of Expression by Dexamethasone in HEK293 Cells

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Abstract

The molecular and cellular basis of the psychotropic actions of adrenal corticosteroids is poorly understood. Previously, we reported that modulation of large conductance Ca2+-activated potassium channel (BK-channel) function by glucocorticoids can be recapitulated in human embryonic kidney293 (HEK293) cells (J Physiol 537:57, 2001). In the present paper, we examined the effect of dexamethasone on the expression of candidate mediator proteins of glucocorticoid action, dex-ras1 and serum and glucocorticoid inducible protein kinase 1 (SGK), in HEK293 cells. Dex-ras1 mRNA was readily detectable under basal conditions however, no changes of dex-ras1 mRNA expression occurred upon exposure to 100 nM of dexamethasone for 2 h. In contrast, a 2.5-fold increase of SGK mRNA was found under similar conditions. Total levels of cellular SGK protein were unaltered upon exposure to dexamethasone, but a marked increase of SGK in a Triton-X100 insoluble fraction was observed. BK-channel α-subunits could not be co-immunoprecipitated with SGK. In summary, SGK, but not dex-ras1, mRNA is rapidly induced by glucocorticoid stimulation in HEK293 cells. However, there appears to be no direct protein-protein interaction between SGK and BK-channel α-subunits.

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Authors and Affiliations

  1. Membrane Biology Group, Centre for Integrative Physiology, University of Edinburgh, Edinburgh, EH8 9XD, UK

    Ghassem Attarzadeh-Yazdi, Michael J. Shipston & Ferenc A. Antoni

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  1. Ghassem Attarzadeh-Yazdi
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  2. Michael J. Shipston
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  3. Ferenc A. Antoni
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Correspondence to Ghassem Attarzadeh-Yazdi.

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Special issue article in honor of George Fink.

Presented to mark the 70th birthday of Professor George Fink.

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Attarzadeh-Yazdi, G., Shipston, M.J. & Antoni, F.A. Dex-ras1 and Serum- and Glucocorticoid-inducible Protein Kinase 1: Regulation of Expression by Dexamethasone in HEK293 Cells. Neurochem Res 33, 609–613 (2008). https://doi.org/10.1007/s11064-007-9516-5

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