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Astroglial-Conditioned Media and Growth Factors Modulate Proliferation and Differentiation of Astrocytes in Primary Culture

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Abstract

Astroglial conditioned media (ACM) influence the development and maturation of cultured nerve cells and modulate neuron-glia interaction. To clarify mechanisms of astroglial cell proliferation/differentiation in culture, incorporation of [methyl-3H]-thymidine or [5,6-3H]-uridine in cultured astrocytes was assessed. Cultures were pre-treated with epidermal growth factor (EGF), insulin (INS), insulin-like growth factor-I (IGF-I), and basic fibroblast growth factor (bFGF) and subsequently with ACM. DNA labeling revealed a marked stimulatory effect of ACM from 15 days in vitro (DIV) cultures in 30 DIV astrocytes after12 h pre-treatment with growth factors. The main effects were found after INS or EGF pre-treatment in 30 DIV cultures. ACM collected from 15 or 60 or 90 DIV increased RNA labeling of 15 and 30 DIV astrocyte cultures, being the highest value that of 30 DIV cultures added with ACM from 90 DIV. The findings of increased DNA labeling after EGF or INS pre-treatment in 30 DIV cultures, followed by addition of ACM from 15 DIV cultures, suggest that these phenomena may depend by extra cellular signal-regulated kinase 1 (ERK1) activation.

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Acknowledgments

Support to RA from the Italian National Research Centre (No. 97.04535.CT04), Italian Ministry of University, Scientific and Technological Research (MURST) (No. 21040184 and 20104030075) and MIUR-COFIN (No. 2001054379) is gratefully acknowledged.

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Authors and Affiliations

  1. Section of Biochemistry and Molecular Biology, Department of Chemical Sciences, University of Catania, Viale Andrea Doria, 6, Catania, Italy

    Vincenzo Bramanti & Roberto Avola

  2. Departments of Biological Chemistry, Medical Chemistry and Molecular Biology, University of Catania, Catania, Italy

    Agata Campisi, Antonino Costa & Marcello Avitabile

  3. Section of Human Anatomy, Department of Experimental Medicine and Public Health, University of Camerino, Camerino, Italy

    Daniele Tomassoni & Francesco Amenta

  4. Section of Urgency and Internal Medicine, Department of Internal Medicine and Systemic Pathologies, University of Catania, Catania, Italy

    Alfredo Fisichella

  5. Section of Human Anatomy, Department “G.F. Ingrassia”, University of Catania, Catania, Italy

    Venera Mazzone

  6. Institute of Neurosurgery, Catholic University of Sacred Heart, Rome, Italy

    Luca Denaro

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  1. Vincenzo Bramanti
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  2. Agata Campisi
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  10. Roberto Avola
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Correspondence to Roberto Avola.

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Bramanti, V., Campisi, A., Tomassoni, D. et al. Astroglial-Conditioned Media and Growth Factors Modulate Proliferation and Differentiation of Astrocytes in Primary Culture. Neurochem Res 32, 49–56 (2007). https://doi.org/10.1007/s11064-006-9224-6

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  • DOI: https://doi.org/10.1007/s11064-006-9224-6

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