Neurochemical Research

, Volume 32, Issue 1, pp 19–25 | Cite as

Spinal Morphine Administration Reduces the Fatty Acid Contents in Spinal Cord and Brain by Increasing Oxidative Stress

  • İsmail Özmen
  • Mustafa NazıroğluEmail author
  • H. Ahmet Alici
  • Fikrettin Şahin
  • Mustafa Cengiz
  • İbrahim Eren
Original Paper


It is well known that oxidative stress damages bimolecules such as DNA and lipids. No study is available on the morphine-induced oxidative damage and fatty acids changes in brain and spinal tissues. The aim of this work was to determine the effects of morphine on the concentrations and compositions of fatty acid in spinal cord segments and brain tissues in rabbits as well as lipid peroxidation (LP) and glutathione (GSH) levels in cortex brain.

Twelve New Zealand albino rabbits were used and they were randomly assigned to two groups of 6 rabbits each. First group used as control although morphine administrated to rats in second group. Cortex brain and (cervical, thoracic, lumbar) samples were taken.

The fatty acids between n:18.0 and 21.0 were present in spinal cord sections and n:10 fatty acids in control animals were present in the brain tissues. Compared to n:20.0–24.0 fatty acids in spinal cord sections and 8.0 fatty acids in the brain tissues of drug administered animals. The concentration and composition of the fatty acid methyl esters in spinal cord and brain tissues was decreased by morphine treatments. LP levels in the cortex brain were increased although GSH levels were decreased by the morphine administration.

In conclusion, unsaturated fatty acids contents in brain and spinal cord sections and GSH were reduced by administrating spinal morphine although oxidative stress as LP increased. The inhibition oxidative damage may be a useful strategy for the development of a new protection for morphine administration as well as opiate abuse.


Morphine Fatty acids Brain Lipid peroxidation Spinal cord Glutathione 



Blood-brain barrier


Fatty acids


Fatty acid methyl esters


Reactive oxygen species


Reduced glutathione


Polyunsaturated fatty acids


Heart rate



The authors thank Ayse Gokce, a technician at Biotechnology Application and Research Center, Erzurum, Turkey.


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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • İsmail Özmen
    • 1
  • Mustafa Nazıroğlu
    • 2
    Email author
  • H. Ahmet Alici
    • 3
  • Fikrettin Şahin
    • 4
  • Mustafa Cengiz
    • 1
  • İbrahim Eren
    • 5
  1. 1.Department of Chemistry, Art and Science FacultySuleyman Demirel UniversityIspartaTurkey
  2. 2.Department of Biophysics, Faculty of MedicineSuleyman Demirel UniversityIspartaTurkey
  3. 3.Department of Anesthesiology and Reanimation, Medical FacultyAtaturk UniversityErzurumTurkey
  4. 4.Department of Genetic and BioengineeringYeditepe UniversityIstanbulTurkey
  5. 5.Department of Psychiatry, Faculty of MedicineSuleyman Demirel UniversityIspartaTurkey

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