The Increase of Choline Acetyltransferase Activity by Docosahexaenoic Acid in NG108-15 Cells Grown in Serum-free Medium is Independent of its Effect on Cell Growth
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We investigated the influence of the polyunsaturated docosahexaenoic acid (22:6n-3; DHA) on the constitutive expression of choline acetyltransferase (ChAT) in native and induced expression in differentiated cholinergic cells NG108-15 grown in serum-free medium. Elimination of serum-derived trophic support resulted in growth arrest and a strong decrease of ChAT activity. In either conditions, DHA largely rescued general indicators of cell growth and function, and partially prevented the decrease of ChAT activity. However, the maximal effect on general cell state in native and differentiated cells, and ChAT activity in native cells, was reached at or below 10 μmol/l of DHA. In contrast, maximal induction of ChAT activity in differentiated cells required about six times higher concentrations of DHA. These data thus demonstrate stimulatory effect of DHA on ChAT activity that is independent of its general cell protective properties.
KeywordsCholinergic neuron Docosahexaenoic acid Trophic support Cell growth Choline acetyltransferase Alzheimer’s disease Calcium influx Oxidative load α-Secretase Caspase-3 Cholesterol
This study was enabled by Research project AV0Z 5011922 supported by EU project QLK1-CT-2002-00172 and grants IAA5011206 and LC554.
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