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Mass Spectrometrical Analysis of Galectin Proteins in Primary Rat Cerebellar Astrocytes

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Abstract

Galectins are a family of animal lectins with specificity for β-galactosides and are involved in a host of cellular activities, ranging from development to cancer. The molecules are expressed by neural and non-neural cells intracellularly as well as extracellularly. Using two-dimensional gel electrophoresis coupled to tandem mass spectrometry, the present work aimed to identify and characterize galectins in primary rat cerebellar astrocytes. The protein-chemical method identified nine spots representing two members of the galectin family, namely galectin-1 and galectin-3. These findings suggest that high abundant expression of galectin in astrocytes is limited to the two abundant galectin family members. As these family members are linked to human astrocytic tumors, their reliable detection in astrocytes by proteomic techniques would enable us to further understand their role in neural development, injury, and regeneration in general and astrocytoma in particular.

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Acknowledgments

Dr. Ephrem Engidawork is grateful for the support of Austrian Embassy Development Cooperation, Addis Ababa, Ethiopia.

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Authors and Affiliations

  1. Department of Paediatrics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria

    J. W. Yang, S. U. Kang, E. Engidawork & G. Lubec

  2. Department of Pharmacology, School of Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia

    E. Engidawork

  3. Laboratory of Neurobiology, Fundación Valenciana de Investigaciones Biomédicas, Valencia, Spain

    R. Rodrigo & V. Felipo

Authors
  1. J. W. Yang
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  2. S. U. Kang
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  3. E. Engidawork
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  5. V. Felipo
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  6. G. Lubec
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Correspondence to G. Lubec.

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Yang, J.W., Kang, S.U., Engidawork, E. et al. Mass Spectrometrical Analysis of Galectin Proteins in Primary Rat Cerebellar Astrocytes. Neurochem Res 31, 945–955 (2006). https://doi.org/10.1007/s11064-006-9100-4

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  • DOI: https://doi.org/10.1007/s11064-006-9100-4

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