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Neurochemical Research

, Volume 31, Issue 3, pp 333–340 | Cite as

Glial Activation and Segmental Upregulation of Interleukin-1β (IL-1β) in the Rat Spinal Cord after Surgical Incision

  • Di Fu
  • Qulian Guo
  • Yuhang Ai
  • Hongwei Cai
  • Jianqin Yan
  • Ruping Dai
Article

Abstract

The present study investigated the expression patterns of glial cells and interleukin-1β (IL-1β) in the rat spinal cord after a surgical incision, which is closely related with clinical postoperative pain. Microglia and astrocytes became activated in the spinal cord following incision. Real-time polymerase chain reaction (PCR) and immunohistochemisty showed that IL-1β mRNA and protein level in the spinal cord was transiently upregulated after surgical incision. The increased IL-1β-immunoreactivity (IR) was mainly localized in neurons but not the activated microglia or astrocytes. Although obvious increase in IL-1β-IR could be observed in the lumbar segments of the spinal cord ipsilateral to a hind paw incision, significant upregulation of IL-1β was not detected in the lumbar segments following thoracic incision. The present study indicated that surgical incision could induce glial activation and segmental upregulation of IL-1β in the spinal cord. The activated glial cells and upregulated IL-1β, in turn, may be involved in the incision-induced pain hypersensitivity.

Keywords

Cytokines Postoperative pain Spinal cord Interleukin-1β (IL-1β) 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Di Fu
    • 1
  • Qulian Guo
    • 1
  • Yuhang Ai
    • 1
  • Hongwei Cai
    • 1
  • Jianqin Yan
    • 1
  • Ruping Dai
    • 1
    • 2
    • 3
  1. 1.Department of Anesthesiology, Xiang-Ya Hospital, Xiang-Ya College of MedicineCentral South UniversityChangshaChina
  2. 2.Gene Expression and Regulatory BiologyInstitute of Molecular and Cell BiologySingaporeSingapore
  3. 3.Department of Anesthesiology, Xiang-Ya Hospital, Xiang-Ya College of MedicineCentral South UniversityChangshaChina

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