Neurochemical Research

, Volume 30, Issue 9, pp 1079–1085 | Cite as

Acrylamide-Induced Changes in the Neurofilament Protein of Rat Cerebrum Fractions

  • Sufang Yu
  • Xiulan Zhao
  • Tianliang Zhang
  • Lihua Yu
  • Shanxia Li
  • Ning Cui
  • Xiaoying Han
  • Zhenping Zhu
  • Keqin Xie


Acrylamide (ACR) is known to produce central–peripheral distal axonopathy, which is characterized by distal swellings and secondary degeneration both in experimental animals and human. Ultrastructurally, excessive accumulation of neurofilaments (NFs) in the distal swollen axon is a major pathological hallmark. However, the mechanisms of ACR axonopathy remain unknown. Twenty seven male Wistar rats were randomly divided into three groups. Lower and higher ACR groups were received 20 and 40 mg/kg ACR by i.p. injection respectively. The control group received physiological saline. All rats were sacrificed after 8 weeks of treatment and their cerebrums were dissected, homogenized and used for the determination of the NF proteins. In general, the levels of light NF (NF-L) and medium NF (NF-M) subunits increased consistently in the supernatant, whereas they decreased consistently in the pellet from rats treated with ACR. Compared to that of the control group, the levels of NF-L increased respectively by 104% and 45% (P<0.01) in the supernatant and decreased by 16% and 11% (P<0.01) in the pellet of rat cerebrums in lower and higher groups. The enhancement of NF-M was 76% and 147% (P<0.05, P<0.01) in supernatant, and the reduction was 26% and 36% (P<0.01) in pellet in lower and higher group respectively. The heavy NF (NF-H) level changed slightly. The present results suggested that the change of NF-L and NF-M levels in cerebrum might be relevant to the mechanisms of the neurofilamentous axonopathies induced by ACR.


Acrylamide axonopathy cerebrum neurofilament protein rat 


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Sufang Yu
    • 1
  • Xiulan Zhao
    • 1
  • Tianliang Zhang
    • 1
    • 2
  • Lihua Yu
    • 1
  • Shanxia Li
    • 1
  • Ning Cui
    • 1
  • Xiaoying Han
    • 1
  • Zhenping Zhu
    • 1
  • Keqin Xie
    • 1
  1. 1.Institute of ToxicologyShandong UniversityJinanP.R. China
  2. 2.Department of ToxicologyShandong Center for Disease Control and PreventionShandong, JinanP.R. China.

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