Neurochemical Research

, Volume 30, Issue 5, pp 603–611 | Cite as

Signal Transduction Mechanisms Involved in the Proliferation of C6 Glioma Cells Induced by Lysophosphatidic Acid

  • Sirlene R. Cechin
  • Peter R. Dunkley
  • Richard Rodnight


We studied pathways involved in the proliferation of rat C6 glioma cells induced by lysophosphatidic acid (LPA), a phospholipid with diverse biological functions. LPA induced a dose–responsive proliferation of C6 cells after 48 h. Proliferation was blocked by inhibitors of the sodium/proton exchanger type 1 (NHE1), Rho-associated kinase, the phosphatidylinositol 3-kinase/Akt pathway (PI3K/Akt), protein kinase C (PKC) and extracellular signal regulated kinase kinase (MEK). Phospho-specific antibodies were used to investigate the pathways involved. LPA induced transient (10 min) phosphorylations of ERK 1/2, Akt and the transcription factor CREB. The LPA-induced phosphorylation of ERK 1/2 and CREB was blocked by inhibition of PI3K, PKC and MEK, but that of Akt was only inhibited by wortmannin, the PI3K inhibitor. Inhibition of Rho kinase or NHE1 did not reduce the LPA-induced phosphorylation of ERK, Akt or CREB. The results were compared with the effects of LPA on transduction pathways in other cell types.


C6 glioma cells lysophosphatidic acid (LPA) sodium/proton exchange enzyme type 1 (NHE1) Rho-associated kinase phosphatidyinositol 3-kinase/Akt CREB ERK 1/2 


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Sirlene R. Cechin
    • 1
    • 2
  • Peter R. Dunkley
    • 1
  • Richard Rodnight
    • 1
    • 2
    • 3
  1. 1.School of Biomedical Sciences and the Hunter Medical Research InstituteUniversity of NewcastleCallaghanAustralia
  2. 2.Departamento de BioquímicaICBS, UFRGSPorto AlegreBrazil
  3. 3.School of Biomedical Science, Medical Sciences BuildingUniversity of NewcastleCallaghanAustralia

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