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Neurochemical Research

, Volume 29, Issue 11, pp 2153–2161 | Cite as

Cytochrome c Release and Caspase-3 Activation in Retinal Ganglion Cells Following Different Distance of Axotomy of the Optic Nerve in Adult Hamsters

  • M.-H. He
  • Z. H. Cheung
  • E.-H. Yu
  • D. K. C. Tay
  • K.-F. So
Article

Abstract

This study examined the relationship between the distance of axotomy and the death of injured retinal ganglion cells (RGCs) in adult hamsters and the relationship of cytochrome c and caspase-3 on the death pathway of RGCs. The left optic nerve (ON) of adult hamsters was transected either at 1 or 3 mm away from the optic disc, and retrogradely labeled with Flurogold on the ON stump. After a predetermined period of postoperative time, the surviving RGCs were counted by retina flat-mount, and the activation of cytochrome c and caspase-3 were investigated by immunohistochemistry. Cell loss was found to be much faster (P < 0.01), more cells with cytochrome c were observed (P < 0.05) and the activation of caspase-3 was earlier when ON was transected 1 mm away from the optic disc than when was transected 3 mm away from the optic disc. Distance of axotomy affects the axotomized cell death rate where more RGCs died when the ON transection was applied closer to the eye. The timing of activation of caspase-3 in the RGCs may be linked to the distance of axotomy.

Keywords

Apoptosis ganglion cells mitochondria optic nerve signal transduction 

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Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • M.-H. He
    • 1
    • 2
  • Z. H. Cheung
    • 1
  • E.-H. Yu
    • 2
  • D. K. C. Tay
    • 1
  • K.-F. So
    • 1
    • 3
  1. 1.Department of AnatomyThe University of Hong KongP.R. China
  2. 2.Department of AnatomyPeking University Health Science centerP.R. China
  3. 3.Department of Anatomy, Faculty of MedicineThe University of Hong KongP.R. China

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