Neurochemical Research

, Volume 30, Issue 2, pp 207–213 | Cite as

Pattern of Glial Fibrillary Acidic Protein Expression Following Kainate-Induced Cerebellar Lesion in Rats

  • I. Milenkovic
  • N. Nedeljkovic
  • R. Filipovic
  • S. Pekovic
  • L. Rakic
  • M. Stojiljkovic

In the present study glial fibrillary acidic protein (GFAP) expression was assessed following intravermian injection of kainic acid (KA) or physiological saline to adult rat cerebellum. After 2- to 30-day recovery period, free-floating sections cut with a microtome were obtained and were proccessed for immunocytochemistry against GFAP. Injection of both kainate and physiological saline elicited significant astrogliotic reaction, i.e. in the area around the lesion thick GFAP-positive Bergmann fibers with typical orientation appeared in the molecular and hypertrophied astrocytes abundantly appeared in the granular layer. However, following kainate intoxication lesion was not surrounded by typical demarcation glial scar during 30-day recovery period in contrast to the appearance of usual glial scar in the group injected with physiological saline, as early as 7-day postlesion. Preserved spatial organization of Bergmann fibers and the absence of typical demarcating glial scar after kainate-induced cerebellar lesion suggest distinct pattern of astrogliosis that presents an interesting model system to study the importance of glial scar in the recovery after ischemic brain insults.


Astrogliosis cerebellum GFAP kainate plasticity rat 


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • I. Milenkovic
    • 1
    • 3
  • N. Nedeljkovic
    • 2
  • R. Filipovic
    • 1
  • S. Pekovic
    • 1
  • L. Rakic
    • 1
  • M. Stojiljkovic
    • 1
    • 2
  1. 1.Department of Neurobiology and NeurochemistryInstitute of Biological Research Sinisa StankovicSerbia and Montenegro
  2. 2.Institute of Physiology and Biochemistry Faculty of BiologyUniversity BelgradeBelgradeSerbia and Montenegro
  3. 3.Department of NeurophysiologyPaul Flechsig Institute of Brain ResearchLeipzigGermany

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