, Volume 44, Issue 6, pp 460–463 | Cite as

Ethanol-Induced Antinociception in Rodents: Role of the Cholinergic and Opioidergic Systems


We examined antinociceptive properties of ethanol and the mechanism of its action using two thermal (hot-plate and tail-flick) and two chemical (acetic acid-induced writhing and formalin) nociception tests. The mechanism of antinociception was analyzed using naloxone (an opioid antagonist) and atropine (a cholinergic blocker). It was found that ethanol in a dose-dependent manner produced significant (P < < 0.05) prolongations of both the hot-plate and tail-flick latencies. In the chemical tests, ethanol caused a significant (P < 0.05) reduction in the number of writhings produced by acetic acid and also a significant decrease (P < 0.01) in the licking time produced by formalin within both phases of the respective test. It was also observed that both atropine and naloxone significantly (P < 0.05) suppressed ethanolinduced antinociception effects. We conclude that the antinociceptive action of ethanol may in part be opiodergic- and cholinergic-dependent.


ethanol pain tests antinociception naloxone atropine 


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© Springer Science+Business Media New York 2012

Authors and Affiliations

  1. 1.University of IbadanIbadanNigeria
  2. 2.Ladoke Akintola University of TechnologyOgbomosoNigeria

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