The induction of development of neurons and glial cells from neural progenitor cells (NPCs) is at present considered a promising strategy for recoverу after ischemic insult-evoked damage to the brain. To estimate whether grafted NPCs can develop morphological properties of the mature neurons and become functionally integrated within a host hippocampal circuitry, immunohistochemical approaches at the light and electron microscopy levels have been used. Ischemic insult in FVB-strain mice was evoked by 20-min-long occlusion of both carotid arteries. One day after occlusion, NPCs from GFP-transgenic fetuses were suboccipitally transplanted into the ischemic brain. We found that 44.7 ± 3.8% (mean ± s.e.m) of the grafted GFP-positive cells differentiated 3 months after transplantation into cells demonstrating morphological features of hippocampal pyramidal neurons. Moreover, grafted cells demonstrated manifestations of rather intense formation of the synapses between host and donor neural cells. Thus, our observations show that the NPC-based transplantation approach may be promising in the treatmen t of ischemic insult.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
R. Kollmar and S. Schwab, “Ischaemic stroke: acute management, intensive care, and future perspectives,” Br. J. Anaesth., 99, No. 1, 95–101 (2007).
K. Jeyaseelan, K. Y. Lim, and A. Armugam, “Neuroprotectants in stroke therapy,” Expert. Opin. Pharmacother., 9, No. 6, 887–900 (2008).
B. A. Williams and A. Keating, “Cell therapy for agerelated disorders: myocardial infarction and stroke – a mini-review,” Gerontology, 54, No. 5, 300–311 (2008).
D. Kondziolka, L. Wechsler, S. Goldstein, et al., “Transplantation of cultured human neuronal cells for patients with stroke,” Neurology, 55, No. 4, 565–569 (2000).
S. I. Savitz, J. H. Dinsmore, L. R .Wechsler, et al., “Cell therapy for stroke,” NeuroRx., 1, No. 4, 406–414 (2004).
S. Haas, N. Weidner, and J. Winkler, “Adult stem cell therapy in stroke,” Curr. Opin. Neurol., 18, No. 1, 59–64 (2005).
B. Jenny, M. Kanemitsu, O. Tsupykov, et al., “Fibroblast growth factor-2 overexpression in transplanted neural progenitors promotes perivascular cluster formation with a neurogenic potential,” Stem Cells, 27, No. 6, 1309–1317 (2009).
V. Darsalia, T. Kallur, and Z. Kokaia, “Survival, migration and neuronal differentiation of human fetal striatal and cortical neural stem cells grafted in stroke-damaged rat striatum,” Eur. J. Neurosci., 26, 605–614 (2007).
M. Wonga, H. Hodgesb, and K. Horsburgh, “Neural stem cell grafts reduce the extent of neuronal damage in a mouse model of global ischaemia,” Brain Res., 1063, 140–150 (2005).
O. M. Tsupykov, T. A. Pivneva, A. O. Poddubna, et al., “Migration and differentiation of fetal neural progenitor cells in the brain of ischemic animals,” Fiziol. Zh., 55, No. 4, 41–49 (2009).
H. Zhang, L. Vutskits, M. S. Pepper, et al., “VEGF is a chemoattractant for FGF-2-stimulated neural progenitors,” J. Cell Biol., 163, 1375–1384 (2003).
H. Toda, J. Takahashi, H. Mizoguchi, et al., “Neurons generated from adult rat hippocampal stem cells form functional glutamatergic and GABAergic synapses in vitro,” Exp. Neurol., 165, 66–76 (2000).
H. Van Praag, A.F. Schinder, B.R. Christie, et al., “Functional neurogenesis in the adult hippocampus,” Nature, 415, 1030–1034 (2002).
Author information
Authors and Affiliations
Corresponding author
Additional information
Neirofiziologiya/Neurophysiology, Vol. 43, No. 4, pp. 372–375, July-August, 2011.
Rights and permissions
About this article
Cite this article
Tsupykov, O.M., Poddubnaya, A.O., Smozhanyk, K.G. et al. Integration of Grafted Neural Progenitor Cells in a Host Hippocampal Circuitry after Ischemic Injury. Neurophysiology 43, 324–326 (2011). https://doi.org/10.1007/s11062-011-9224-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11062-011-9224-2