Modulation of Glycinergic Transmission in the Rat Spinal Dorsal Commissural Nucleus by Ginkgolide B
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The action of ginkgolide B (GB), a powerful compound of Ginkgo biloba extract, on glycine-mediated spontaneous currents in rat spinal sacral dorsal commissural nucleus (SDCN) neurons was examined. IPSCs evoked in spinal cord slices were inhibited in a dose-dependent manner by the addition of GB to the superfusion solution. The amplitude of eIPSCs was reduced to 61 ± 6.4% by 10 μM GB with acceleration of the kinetics of the currents, indicating the effect of GB on channel pores. Both the amplitude and success ratio (Rsuc) of eIPSC induced by electrical focal stimulation of single glycinergic nerve endings (boutons) also changed in the presence of 1 μM GB. These data suggest that GB modulates not only post-synaptic glycine receptors but also the pre-synaptic glycine release machinery.
Keywordsspinal glycine receptor evoked inhibitory postsynaptic current (eIPSC) presynaptic bouton focal stimulation ginkgolide B
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- 5.K. Hosl, H. Reinold, R. J. Harvey, et al., “Spinal prostaglandin E receptors of the Ep2 subtype and the glycine receptor alpha3 subunit, which mediate central inflammatory hyperalgesia, do not contribute to pain after peripheral nerve injury or formalin injection,” Pain, 126, 46-53 (2006).CrossRefPubMedGoogle Scholar
- 6.J. W. Lynch and R. J. Callister, “Glycine receptors: a new therapeutic target in pain pathways,” Curr. Opin. Invest. Drugs, 7, 48-53 (2006).Google Scholar