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Neurophysiology

, 41:157 | Cite as

Role of Glutamate in the Mechanisms of Adaptation of the System of Respiratory Control in Rats to Intermittent Hypoxia

  • E. É. Kolesnikova
  • V. I. Nosar
  • I. N. Mankovskaya
Article

In experiments on Wistar rats, we studied the role of changes in the state of glutamatergic transmission in the course of adaptation of the system of respiratory control to intermittent hypoxia. The volume/temporal parameters of respiration were estimated according to characteristics of EMG activity (amplitude, integral intensity of EMG discharges) recorded from the diaphragmatic muscle. Changes in EMG activity of the diaphragm induced by acute hypoxia (breathing a 12% О2-containing gas mixture) were estimated before and after of a 14-day-long course of intermittent hypoxia trainings and before and after inductions of a blocker of NMDA receptors, МK-801. The results prove that the glutamatergic transmitter system is significantly involved in the reaction of the respiratory system to presentation of a hypoxic stimulus within all stages of formation of the ventilatory response, both before and after the action of intermittent hypoxia. Blocking of NMDA receptors under conditions of adaptation to intermittent hypoxia exerted a more intense influence on the amplitude of respiratory EMG discharges of the diaphragm than on their frequency.

Keywords

intermittent hypoxia EMG activity diaphragm brainstem glutamate MK-801 

References

  1. 1.
    E. A. Aaron and F. L. Powell, “Effect of chronic hypoxia on hypobaric ventilatory responses in awake rats,” J. Appl. Physiol., 74, 1635-1640 (1993).PubMedGoogle Scholar
  2. 2.
    G. E. Bisgard and J. A. Neubauer, “Peripheral and central effects of hypoxia,” in: Lung Biology in Health and Disease, Marcel Dekker, New York (1995), pp. 617-668.Google Scholar
  3. 3.
    T. V. Serebrovskaya, “Intermittent hypoxia research in the former Soviet Union and the Commonwealth of Independent States (CIS): history and review of the concept and selective application,” High Altitude Biol., 3, 205-221 (2002).CrossRefGoogle Scholar
  4. 4.
    R. C. Ang, B. Hoop, and H. Kazemi, “Role of glutamate as the central neurotransmitter in the hypoxic ventilatory response,” J. Appl. Physiol., 72, 1480-1487 (1992).PubMedGoogle Scholar
  5. 5.
    H. Kazemi, C. H. Chiang, and B. Hoop, “Role of medullary glutamate in the hypoxic ventilatory response,” in: Comroe Memorial Symposium – Chemoreceptors and Reflex in Breathing, S. Lahiri (ed.), Oxford Univ. Press, New York (1989), pp. 233-242.Google Scholar
  6. 6.
    B. Hoop, M.-R. Masjedi, V. E. Shin, and H. Kazemi, “Brain glutamate metabolism during hypoxia and peripheral chemodenervation,” J. Appl. Physiol., 69, 147-154 (1990).PubMedGoogle Scholar
  7. 7.
    P. J. Ohtake, J. E. Torres, Y. M. Gozal, et al., “NMDA receptors mediate peripheral chemoreceptor afferent input in the conscious rat,” J. Appl. Physiol., 84, 853-861 (1998).PubMedGoogle Scholar
  8. 8.
    S. G. Reid and F. L. Powell, “Effects of chronic hypoxia on MK-801-induced changes in the acute hypoxic ventilatory response,” J. Appl. Physiol., 99, 208-2114 (2005).CrossRefGoogle Scholar
  9. 9.
    M. Pokorski, E. Kolesnikova, M. Marczak, and K. Budzinska, “Neurotransmitter mechanisms in the enhancement of the hypoxic ventilatory response by antecedent hyperoxia in the anesthetized rat,” J. Physiol. Pharmacol., 56, 433-446 (2005).PubMedGoogle Scholar
  10. 10.
    D. T. Monaghan and C. W. Cotman, “Distribution of N-methyl-D-aspartate-sensitive L-[3H]glutamate-binding sites in rat brain,” J. Neurosci., 5, 2909-2919 (1985).PubMedGoogle Scholar
  11. 11.
    D. W. Richter, P. Schmidt-Garson, O. Pierrefiche, et al., “Neurotransmitters and neuromodulators controlling the hypoxic ventilatory response in anaesthetized cats,” J. Physiol., 514, 567-578 (1999).CrossRefPubMedGoogle Scholar
  12. 12.
    C. H. Chiang, P. Pappagianopulos, B. Hoop, and H. Kazemi, “Central cardiorespiratory effects of glutamate in dogs,” J. Appl. Physiol., 60, 2056-2062 (1986).PubMedGoogle Scholar
  13. 13.
    S. K. Coles, P. Ernsbergher, and T. E. Dick, “A role for NMDA receptors in posthypoxic frequency decline in the rat,” Am. J. Physiol. Regul. Integr. Comp. Physiol., 274, R1546-R1555 (1998).Google Scholar
  14. 14.
    A. Mizusawa, H. Ogawa, Y. Kikuchi, et al., “In vivo release of glutamate in nucleus tractus solitarii of the rat during hypoxia,” J. Physiol., 478.1, 55-65 (1994).Google Scholar
  15. 15.
    I. Tarakanov, A. Dymecka, and M. Pokorski, “NMDA glutamate receptor antagonism and the ventilatory response to hypoxia in the anesthetized rat,” J. Physiol. Pharmacol., 55, Suppl. 3, 139-147 (2004).PubMedGoogle Scholar
  16. 16.
    S. Kobayashi and D. E. Millhorn, “Regulation of N-methyl-D-aspartate receptor expression and N-methyl-D-aspartate-induced cellular response during chronic hypoxia in differentiated PC12 cells,” Neuroscience, 101, 1153-1162 (2000).CrossRefPubMedGoogle Scholar
  17. 17.
    S. R. Reeves, E. Gozal, S. Z. Guo, et al., “Effect of long-term intermittent and sustained hypoxia on hypoxic ventilatory and metabolic response in adult rats,” J. Appl. Physiol., 95, 1767-1774 (2003).PubMedGoogle Scholar
  18. 18.
    N. Simakajornboon, G. R. Graff, J. E. Torres, and D. Gozal, “Modulation of hypoxic ventilatory response by systemic platelet-activating factor receptor antagonist in the rat,” Respir. Physiol., 114, 213-225 (1998).CrossRefPubMedGoogle Scholar
  19. 19.
    P. Schmitt, V. Soulier, J. M. Pequignot, et al., “Ventilatory acclimatization to chronic hypoxia: relationship to noradrenaline metabolism in the rat solitary complex,” J. Physiol., 477, 331-337 (1994).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • E. É. Kolesnikova
    • 1
  • V. I. Nosar
    • 1
  • I. N. Mankovskaya
    • 1
  1. 1.Bogomolets Institute of PhysiologyNational Academy of Sciences of UkraineKyivUkraine

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