Inhibitory drives in the nervous system are provided by synapses operating mostly through Cl− ion channels. We describe a novel CFP-YFP-based fluorescence Cl− indicator, Cl-sensor, characterized by a high sensitivity, which can be successfully used as a tool for monitoring intracellular Cl− in various biological preparations.
This is a preview of subscription content, log in to check access.
O. P. Hamill, J. Bormann, and B. Sakmann, “Activation of multiple-conductance state chloride channels in spinal neurones by glycine and GABA,” Nature, 305, 805 (1983).PubMedCrossRefGoogle Scholar
T. Kuner and G. J. Augustine, “A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons,” Neuron, 27, 447 (2000).PubMedCrossRefGoogle Scholar
R. Tyzio, R. Cossart, I. Khalilov, et al., “Maternal oxytocin triggers a transient inhibitory switch in GABA signaling in the fetal brain during delivery,” Science, 314, 1788 (2006).PubMedCrossRefGoogle Scholar
T. Matsuda and C. L. Cepko, “Electroporation and RNA interference in the rodent retina in vivo and in vitro,” Proc. Natl. Acad. Sci. USA, 101, 16 (2004).PubMedCrossRefGoogle Scholar