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Neurophysiology

, Volume 39, Issue 4–5, pp 332–333 | Cite as

A genetically encoded indicator for monitoring intracellular chloride

  • O. Markova
  • E. Réal
  • Y. Jacob
  • M. Mukhtarov
  • P. Bregestovski
Article

Abstract

Inhibitory drives in the nervous system are provided by synapses operating mostly through Cl ion channels. We describe a novel CFP-YFP-based fluorescence Cl indicator, Cl-sensor, characterized by a high sensitivity, which can be successfully used as a tool for monitoring intracellular Cl in various biological preparations.

Keywords

fluorescent probes real-time optical detection patch clamp 

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • O. Markova
    • 1
    • 3
  • E. Réal
    • 2
  • Y. Jacob
    • 2
  • M. Mukhtarov
    • 1
  • P. Bregestovski
    • 1
  1. 1.Institut de Neurobiologie de la Méditerranée (INMED)MarseilleFrance
  2. 2.Pasteur InstituteParisFrance
  3. 3.Bogomolets Institute of Physiology National Academy of Sciences of UkraineKyivUkraine

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