Abstract
Inhibitory drives in the nervous system are provided by synapses operating mostly through Cl− ion channels. We describe a novel CFP-YFP-based fluorescence Cl− indicator, Cl-sensor, characterized by a high sensitivity, which can be successfully used as a tool for monitoring intracellular Cl− in various biological preparations.
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References
O. P. Hamill, J. Bormann, and B. Sakmann, “Activation of multiple-conductance state chloride channels in spinal neurones by glycine and GABA,” Nature, 305, 805 (1983).
T. Kuner and G. J. Augustine, “A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons,” Neuron, 27, 447 (2000).
R. Tyzio, R. Cossart, I. Khalilov, et al., “Maternal oxytocin triggers a transient inhibitory switch in GABA signaling in the fetal brain during delivery,” Science, 314, 1788 (2006).
T. Matsuda and C. L. Cepko, “Electroporation and RNA interference in the rodent retina in vivo and in vitro,” Proc. Natl. Acad. Sci. USA, 101, 16 (2004).
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Neirofiziologiya/Neurophysiology, Vol. 39, Nos. 4/5, pp. 380–381, July–October, 2007.
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Markova, O., Réal, E., Jacob, Y. et al. A genetically encoded indicator for monitoring intracellular chloride. Neurophysiology 39, 332–333 (2007). https://doi.org/10.1007/s11062-007-0047-0
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DOI: https://doi.org/10.1007/s11062-007-0047-0