, Volume 39, Issue 4–5, pp 305–306 | Cite as

Visualizing recycling of synaptic vesicle proteins

  • J. Klingauf


The use of modern techniques (in particular, novel fluorescence markers of a few molecular participants of the exo-and endocytotic processes, including pH-sensitive agents, immuno-electron and laser-scanning microscopy) allows experimenters to visualize different stages of recycling of synaptic vesicle proteins.


synaptic vesicle recycling exocytosis endocytosis 


  1. 1.
    J. E. Heuser and T. S. Reese, “Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction,” J. Cell Biol., 57, No. 2, 315–344 (1973).PubMedCrossRefGoogle Scholar
  2. 2.
    B. Ceccarelli, W. P. Hurlbut, and A. Mauro, “Turnover of transmitter and synaptic vesicles at the frog neuromuscular junction,” J. Cell Biol., 57, No. 2, 499–524 (1973).PubMedCrossRefGoogle Scholar
  3. 3.
    K. I. Willig et al., “STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis,” Nature, 440, No. 7086, 935–939 (2006).PubMedCrossRefGoogle Scholar
  4. 4.
    M. Wienisch and J. Klingauf, “Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical,” Nat. Neurosci., 9, No. 8, 1019–1027 (2006).PubMedCrossRefGoogle Scholar
  5. 5.
    T. Fernandez-Alfonso, R. Kwan, and T. A. Ryan, “Synaptic vesicles interchange their membrane proteins with a large surface reservoir during recycling,” Neuron, 51, No. 2, 179–186 (2006).PubMedCrossRefGoogle Scholar
  6. 6.
    J. S. Dittman and J. M. Kaplan, “Factors regulating the abundance and localization of synaptobrevin in the plasma membrane,” Proc. Natl. Acad. Sci. USA, 103, No. 30, 11399–11404 (2006).PubMedCrossRefGoogle Scholar
  7. 7.
    G. Miesenbock, D. A. De Angelis, and J. E. Rothman, “Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins,” Nature, 394, No. 6689, 192–195 (1998).PubMedCrossRefGoogle Scholar
  8. 8.
    S. Sankaranarayanan et al., “The use of pHluorins for optical measurements of presynaptic activity,” Biophys. J., 79, No. 4, 2199–2208 (2000).PubMedCrossRefGoogle Scholar
  9. 9.
    P. Taubenblatt et al., “VAMP (synaptobrevin) is present in the plasma membrane of nerve terminals,” J. Cell Sci., 112, Part 20, 3559–3567 (1999).PubMedGoogle Scholar
  10. 10.
    E. A. Lemke and J. Klingauf, “Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity,” J. Neurosci., 25, No. 47, 11034–11044 (2005).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  1. 1.Max-Planck Institut Biophysikalische ChemieGüttingenGermany

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