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MiR-101-3p inhibits EMT to attenuate proliferation and metastasis in glioblastoma by targeting TRIM44

  • Laboratory Investigation
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A Correction to this article was published on 22 September 2023

This article has been updated

Abstract

Background

Glioblastoma (GBM) is the most common malignant tumor originating in the brain parenchyma. The invasive and infiltrative properties of glioblastoma result in poor clinical prognosis to conventional therapies. Emerging reports on microRNAs as important regulators during the process of EMT provide new insights into treating glioblastoma through new targets. However, underlying molecular mechanism of the regulation of miR-101-3p in glioblastoma remains unclear.

Methods

Level of miR-101-3p was determined in GBM cell lines by qRT-PCR. MTT, colony formation and transwell assays were utilized to evaluate functions of overexpression of miR-101-3p/knock down of TRIM44 on proliferation, migration and invasion in GBM cells. Direct interaction between miR-101-3p and TRIM44 was validated using dual luciferase reporter system and impacts of overexpression of miR-101-3p/knock down of TRIM44 on regulation of EMT markers were assessed by Western blotting.

Results

MiR-101-3p was validated to be repressed expressed in glioblastoma cancer cell lines. Both overexpression of miR-101-3p and knock down of TRIM44 attenuated proliferation, migration and invasion of glioblastoma cell lines in vitro. TRIM44 was shown to promote EMT in GBM progress and reverse inhibitory function of miR-101-3p. MiR-101-3p was found to suppress the expression of TRIM44 via directly targeting its 3′UTR.

Conclusions

Our findings suggested miR-101-3p regulated proliferation and migration of glioblastoma cells through attenuating TRIM44 induced EMT via direct targeting 3′UTR of TRIM44, which provided preliminary study of potential therapeutic target in future GBM treatment.

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Data availability

Statistical analysis was performed by GraphPad Prism software version 6.0 (GraphPad Software Inc, USA).

Change history

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Acknowledgements

We would like to give our sincere gratitude to the reviewers for their constructive comments.

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Authors and Affiliations

  1. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, No. 87, Xiangya Road, Kaifu District, Changsha, 410008, Hunan, People’s Republic of China

    Ling Li, Mei-Ying Shao, Zhe-Feng Xiao & Zhu-Chu Chen

  2. Department of Neurosurgery, Brain Hospital of Hunan Province, Changsha, 410008, People’s Republic of China

    Ling Li & Shu-Cheng Zou

Authors
  1. Ling Li
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  2. Mei-Ying Shao
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  3. Shu-Cheng Zou
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  4. Zhe-Feng Xiao
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Contributions

LL: Guarantor of integrity of the entire study, LL, SMY: study concepts, XZF, CZC: study design, LL, ZSC: definition of intellectual content, SMY, ZSC: literature research, SMY, XZF: clinical studies, SMY, XZF: experimental studies, CZC: data acquisition, CZC, ZSC: data analysis, XZF, CZC: statistical analysis, XZF, CZC: manuscript preparation, LL, SMY: manuscript editing, LL: manuscript review.

Corresponding authors

Correspondence to Zhe-Feng Xiao or Zhu-Chu Chen.

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Conflict of interest

The authors declare that they have no competing interests.

Additional information

The original version of this article has been revised: Figures 2A, 2C, 2E, 5A, 5c, 5E and 6C have been corrected.

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Li, L., Shao, MY., Zou, SC. et al. MiR-101-3p inhibits EMT to attenuate proliferation and metastasis in glioblastoma by targeting TRIM44. J Neurooncol 141, 19–30 (2019). https://doi.org/10.1007/s11060-018-2973-7

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  • DOI: https://doi.org/10.1007/s11060-018-2973-7

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