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Magnetic resonance perfusion-weighted imaging defines angiogenic subtypes of oligodendroglioma according to 1p19q and EGFR status

  • Lab Investigation - human/animal tissue
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Abstract

1p19q LOH has been shown to predict radio- and chemosensitivity and prolonged survival in oligodendrogliomas (OLs). We have recently shown that magnetic resonance perfusion-weighted imaging (MR-PWI) may be useful in predicting the histopathological grade or cytogenetic type of oligodendroglial neoplasms. MR-PWI allows noninvasive determination of relative tumor blood volume (rTBV), which may reflect the degree of neoplastic angiogenesis and metabolism. The present study was aimed to correlate rTBV to the angiogenic markers and EGFR expression in oligodendroglial tumors with 1p/19q LOH or 1p LOH (Group 1) and 1p19q intact alleles or 19q LOH (Group 2), respectively. In WHO grade II neoplasms, Group 1 showed significantly greater rTBV than Group 2 (P = 0.013). However, the differences between Group 1 and Group 2 were not significant in grade III tumors. Probe-based real-time RT-PCR analyses showed that 12% of Group 2 high-grade tumors with intact 1p19q exhibited dramatic EGFR overexpression (designated EGFR-high). Grade III neoplasms showed a significantly higher rTBV than grade II neoplasms. Group 1 tumors showed significantly higher rTBV than Group 2 tumors, independent of the EGFR-high subtype. Real-time RT-PCR analyses showed increased expression of VEGF, CD31 and CD105 in Group 1 tumors as compared to Group 2 tumors, excluding the EGFR-high subtype. Multivariable linear regression analysis showed a significant association of rTBV with 1p19q LOH, and expression of EGFR and VEGF. Therefore, the combined use of extensive molecular profiling and advanced MR imaging modalities may improve the accuracy of tumor grading, provide prognostic information, and has the potential to influence treatment decisions.

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Acknowledgments

This work was supported by grants to Donald M. O’Rourke from the National Institutes of Health R01 CA-90586, the Department of Veterans Affair (Merit Review Program), The Brain Tumor Society, and the “For Pete’s Sake” Trust fund for oligodendroglioma research.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Pennsylvania, 3 Silverstein 3400 Spruce Street, Philadelphia, PA, 19104, USA

    Gurpreet S. Kapoor, Timothy A. Gocke, Robert G. Whitmore, Joanna Lopinto, Justin Plaum, Eileen Maloney-Wilensky, Kevin D. Judy & Donald M. O’Rourke

  2. Department of Radiology, University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA, 19104, USA

    Sanjeev Chawla, Ali Nabavizadeh, Jaroslaw Krejza, Harish Poptani & Elias R. Melhem

  3. Department of Nuclear Medicine, Medical University of Gdansk, 15 Tuwima Street, 80-210, Gdansk, Poland

    Jaroslaw Krejza

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  1. Gurpreet S. Kapoor
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  2. Timothy A. Gocke
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  3. Sanjeev Chawla
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  12. Kevin D. Judy
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  13. Donald M. O’Rourke
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Corresponding author

Correspondence to Donald M. O’Rourke.

Additional information

Gurpreet S. Kapoor, Timothy A. Gocke and Sanjeev Chawla contributed equally to this study.

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Kapoor, G.S., Gocke, T.A., Chawla, S. et al. Magnetic resonance perfusion-weighted imaging defines angiogenic subtypes of oligodendroglioma according to 1p19q and EGFR status. J Neurooncol 92, 373–386 (2009). https://doi.org/10.1007/s11060-009-9880-x

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  • DOI: https://doi.org/10.1007/s11060-009-9880-x

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