Journal of Neuro-Oncology

, Volume 83, Issue 3, pp 259–266 | Cite as

Expression of VEGF and its receptor genes in intracranial schwannomas

  • Toshio Uesaka
  • Tadahisa Shono
  • Satoshi O. Suzuki
  • Akira Nakamizo
  • Hiroaki Niiro
  • Masahiro Mizoguchi
  • Toru Iwaki
  • Tomio Sasaki
Laboratory Investigation


Vascular endothelial growth factor (VEGF) is considered to be a major regulator of angiogenesis in various brain tumors. In this study, we determined the expression levels of VEGF, and vascular endothelial growth factor receptor (VEGFR)-1 and -2 mRNA in 46 intracranial schwannomas by quantitative real-time PCR, and correlated these with various clinical factors or other molecular markers. We found that these tumors expressed significant amounts of VEGF mRNA in comparison with other brain tumors, including malignant gliomas and meningiomas. In addition, we performed immunohistochemical studies for VEGF and VEGFR-1, and confirmed that these tumors prominently express these proteins. The expression levels of VEGF and VEGFR-1 mRNA in recurrent tumors were higher than those in primary tumors. When we divided patients into two groups according to VEGF mRNA expression in the tumor, there was no significant difference in patient age, gender, or cranial nerves of origin between groups; however, the tumor volume tended to be larger in the high VEGF group than in the low VEGF group. The levels of VEGFR-1 mRNA and neurofibromatosis-2 mRNA in the high VEGF group were significantly greater than those in the low VEGF group. Levels of VEGFR-2 mRNA and DNA topoisomerase IIα mRNA, and the MIB-1 labeling index in the high VEGF group were slightly higher than those in the low VEGF group; however, the difference was not statistically significant. Based on these observations, the significance of VEGF and its receptor genes in intracranial schwannomas is discussed.


Intracranial schwannoma mRNA Real-time PCR VEGF VEGFR-1 VEGFR-2 



We thank Tomomi Yamada, MS (Department of Medical Information Science, Kyushu University Hospital) for her invaluable assistance in statistical analysis, and Saori Uchida for her excellent technical assistance, Mikiko Hidaka and Emiko Uchiyama (Department of Neurosurgery, Kyushu University Graduate School of Medical Sciences) for assistance with manuscript preparation.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Toshio Uesaka
    • 1
  • Tadahisa Shono
    • 1
  • Satoshi O. Suzuki
    • 2
  • Akira Nakamizo
    • 4
  • Hiroaki Niiro
    • 3
  • Masahiro Mizoguchi
    • 1
  • Toru Iwaki
    • 2
  • Tomio Sasaki
    • 1
  1. 1.Department of Neurosurgery, Graduate School of Medical SciencesKyushu UniversityFukuokaJapan
  2. 2.Department of Neuropathlogy, Graduate School of Medical SciencesKyushu UniversityFukuokaJapan
  3. 3.Center for Cellular and Molecular MedicineKyushu University HospitalFukuokaJapan
  4. 4.Department of Neurosurgery, National Hospital OrganizationKyushu Medical CenterFukuokaJapan

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