Journal of Neuro-Oncology

, Volume 77, Issue 3, pp 233–240 | Cite as

Destruction of human retinoblastoma after treatment by the E variant of encephalomyocarditis virus

  • Masazumi Adachi
  • Steven E. Brooks
  • Maxine R. Stein
  • Bernice E. Franklin
  • Francis A. Caccavo
Laboratory Investigation


The oncolytic effects of encephalomyocarditis (EMC) virus were examined in human retinoblastoma cell (Y79) cultures which were infected with 104 tissue culture infectious doses (TCIDs) of the E variant of EMC (EMC-E) virus. The TCIDs were used to titer the maximum effect of EMC virus on L-929 cells. In-vitro studies showed 90% cytopathic effect (CPE) at 24 h and 100% CPE at 52 h. The CPE was used to observe pathologic effects of the cells. In-vivo studies employing human retinoblastoma grown as a tumor in nude mice, revealed degeneration of 80% of the tumor cells at 3 days and total destruction at 4 days following inoculation with the EMC-E virus. The virus is highly neurotropic in mice, but is usually not pathogenic in man. These studies suggest a possible new direction in the treatment of retinoblastoma and other malignant tumors using the viral technology.


encephalomyocarditis virus oncolysis retinoblastoma specific molecular receptor virus inoculation 


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Copyright information

© Springer 2005

Authors and Affiliations

  • Masazumi Adachi
    • 1
    • 2
    • 3
  • Steven E. Brooks
    • 1
  • Maxine R. Stein
    • 1
  • Bernice E. Franklin
    • 1
  • Francis A. Caccavo
    • 1
  1. 1.Isaac Albert ResearchInstitute of Kingsbrook Jewish Medical CenterBrooklynUSA
  2. 2.Department of PathologyState University of New York, Downstate Medical CenterBrooklynUSA
  3. 3.Department of LaboratoriesKingsbrook Jewish Medical CenterBrooklynUSA

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