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Journal of Neuro-Oncology

, Volume 71, Issue 3, pp 231–236 | Cite as

T cell response to Hu-D peptides in patients with anti-Hu syndrome

  • A. Rousseau
  • B. Benyahia
  • J. Dalmau
  • F. Connan
  • J. -G. Guillet
  • J. -Y. Delattre
  • J. Choppin
Laboraatory Investigation

Abstract

The anti-Hu syndrome is the most common paraneoplastic neurologic syndrome but the exact mechanism of immune mediated neuronal injury remains unknown. Anti-Hu antibodies do not appear to play a pivotal role in the pathogenesis of the disease. To assess cell-mediated immunity, we selected 51 peptides from the Hu-D sequence and tested their ability to bind to six common HLA class I molecules. Stable complexes with purified HLA molecules were obtained with 19/51 (37%) selected peptides. Subsequently, the ability of the 19 HLA-binding peptides to stimulate T cells from 10 patients and 10 control subjects was evaluated by detecting IFN-γ secretion. An anti-peptide T-cell response was observed in 7/10 Hu-positive patients but also in 3/10 control subjects. Overall, a significant T-cell activation occurred in response to 74% (14 out of 19) of the selected peptides in the Hu-positive patients vs. 16% (3 out of 19) in the control group (p < 0.001). In addition, T cells of patients tested within 3 months of the onset of anti-Hu syndrome responded to 82% (14 out of 17) of assessed Hu-D peptides vs. 37% (7 out of 19) in patients tested 1 year or more after developing the syndrome (p < 0.01). Thus, the present study suggests a role of cellular immunity during the course of anti-Hu syndrome.

Keywords

anti-Hu syndrome cellular immunity ELISPOT assay HLA/peptide complexes Hu-Depitopes IFN-γ paraneoplastic disorders T lymphocytes 

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Copyright information

© Springer 2005

Authors and Affiliations

  • A. Rousseau
    • 1
  • B. Benyahia
    • 1
  • J. Dalmau
    • 2
  • F. Connan
    • 3
  • J. -G. Guillet
    • 3
  • J. -Y. Delattre
    • 1
  • J. Choppin
    • 3
  1. 1.Department of Neurology and INSERM U495Groupe Hospitalier Pitié-SalpêtrièreParisFrance
  2. 2.Department of NeurologyUniversity of Arkansas for Medical SciencesArkansasUSA
  3. 3.Department of Immunology, Institut Cochin, INSERM U567, CNRS UMR 8104René Descartes UniversityParisFrance

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