New Forests

, Volume 46, Issue 2, pp 309–317 | Cite as

Cold storage of initial plant material affects positively somatic embryogenesis in Pinus radiata

  • I. A. Montalbán
  • O. García-Mendiguren
  • T. Goicoa
  • M. D. Ugarte
  • P. Moncaleán
Short Communication


Traditionally, many efforts have been carried out in order to improve the success of somatic embryogenesis process in conifers, but little attention has been paid to the influence of the plant material storage conditions in the subsequent phases of the somatic embryogenesis process. In this work our objective was to study the feasibility of storing Pinus radiata plant material at 4 °C for long periods in order to make easier the initiation with high amount of cell lines. The effect of cold storage on the different stages of somatic embryogenesis process has been evaluated. Storage periods of 1–3 months enhanced initiation rates and the number of somatic embryos obtained in the embryogenic cell lines. These results demonstrate the beneficial effect of cold storage and open the possibility of considering a cold preconditioning of plant material as a good alternative to improve the somatic embryogenesis process in conifers.


Conifer In vitro Micropropagation Radiata pine Somatic embryo 



This work was supported by Departamento de Desarrollo Económico y Competitividad-Gobierno Vasco-Spain (SOMAPIN-61.0369.0) and Olatz García-Mendiguren PhD scholarship. The work by Goicoa, T. and Ugarte, M.D. was supported by the Spanish Ministry of Science and Innovation (project MTM 2011-22664 which is cofounded by FEDER grants).


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • I. A. Montalbán
    • 1
  • O. García-Mendiguren
    • 1
  • T. Goicoa
    • 2
  • M. D. Ugarte
    • 2
  • P. Moncaleán
    • 1
  1. 1.Neiker-TecnaliaVitoria-GasteizSpain
  2. 2.Dpto. Estadística e Investigación OperativaUniversidad Pública de NavarraPamplonaSpain

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