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Topophysis in Corymbia torelliana × C. citriodora seedlings: adventitious rooting capacity, stem anatomy, and auxin and abscisic acid concentrations

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Abstract

Low amenability of the eucalypt, Corymbia torelliana × C. citriodora, to vegetative propagation has limited its establishment in plantations. This study determined whether rooting capacity and cutting vigour varied along the central shoot of Corymbia seedlings, and whether these differences are related to stem development and hormone concentrations. Dual-node cuttings were harvested from five positions (nodes 1/2, 3/4, 5/6, 7/8 and 9/10) along the seedling shoot (S1), and cuttings were treated with one of four levels of indole-3-butyric acid (IBA). Root formation, root and shoot growth, stem anatomy, and the concentrations of indole-3-acetic (IAA) and abscisic acid (ABA) were assessed at each shoot position. The central node of the rooted cuttings was harvested for fresh cuttings to observe whether maturation emerged in ramets (S2) from the different ortet positions. Rooting and vigour were highest from the most-apical seedling nodes (7/8 and 9/10 in S1), which had less lignification and sclerenchyma development than more-basal nodes (1/2, 3/4 and 5/6). IAA and ABA concentrations differed little between the seedling nodes. In contrast, cuttings from the ramets (in S2) had similar rooting, vigour, lignification and sclerification to each other, but there were large differences in IAA and ABA concentrations depending on their previous point-of-origin in the seedling. High doses of IBA reduced rooting. Rooting and vigour were related to lignification and sclerenchyma development rather than IAA or ABA concentrations. Mass vegetative propagation of C. torelliana × C. citriodora is achievable because mean rooting percentages in S1 and S2 were 61 and 68 %, respectively, using cuttings from small seedling ortets and their ramets.

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Acknowledgments

We thank Tracey McMahon and Pip Bryant for assistance and David Lee (Agri-Science Queensland) for providing seeds. This work was supported by the Brazilian Agricultural Research Corporation (Embrapa) and the Queensland Plantation Hardwoods Research Fund.

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Authors and Affiliations

  1. Embrapa Florestas, Colombo, PR, 83411-00, Brazil

    Ivar Wendling

  2. Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia

    Peter R. Brooks & Stephen J. Trueman

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  1. Ivar Wendling
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Correspondence to Ivar Wendling.

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Wendling, I., Brooks, P.R. & Trueman, S.J. Topophysis in Corymbia torelliana × C. citriodora seedlings: adventitious rooting capacity, stem anatomy, and auxin and abscisic acid concentrations. New Forests 46, 107–120 (2015). https://doi.org/10.1007/s11056-014-9451-7

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